Highlighter stains can be removed with a laundry pre-treatment solution, washing the fabric using a laundry detergent or with rubbing alcohol, according to DoItYourself.com. As with any stain, speed of treatment is of paramount importance.
The first thing to do is to press a white paper towel gently onto the stain, in order to soak up any excess ink in the fabric. The stain can then be treated with any one of the pre-treatment solutions available on the market. The treatment should be applied over the affected area and left to sit for 10 to 15 minutes.
If the stain does not go away, laundry detergent can be rubbed over it. The detergent is then rinsed, and in the case of persistent stains, the rubbing and rinsing may need to be repeated. If all of the steps fail to get rid of the stain, DoItYourself.com recommends using rubbing alcohol. Pour a small amount of rubbing alcohol on the stained fabric while blotting the stain using a white cloth, to soak up the highlighter ink. Rubbing alcohol is naturally very strong, and could damage the fabric, so it is best to use it cautiously. Nail polish remover and hairspray can also be used.