What is gas liquid chromatography?


Gas liquid chromatography involves the vaporization and injection of a sample onto the top of the chromatographer's column. After that, the sample goes through the column as a result of the flow of mobile gases, while the column itself has a stationary liquid phase that adsorbs onto an inert solid surface. At this point, several different detectors can be used, yielding different sorts of selectivity.

It is important for the carrier gas to be inert chemically, and gases that are frequently used include helium, nitrogen, carbon dioxide and argon; the specific choice generally depends on the type of detector in use.

The sample should enter the column as a vapor "plug," as a slower injection of larger samples weakens resolution and broadens the band. Most commonly, a microsyringe injects sample into a vaporizer port through a rubber septum at the column head.

To create precise outcomes, it is important to control temperatures within the column within tenths of a degree, but the optimal temperature varies with the sample's boiling point. A temperature just above the average excess of the sample yields elution time ranging between two or three minutes to half an hour. Samples that have a wide boiling range tend to respond well to temperature programming.

Q&A Related to "What is gas liquid chromatography?"
Gas-liquid chromatography is an analytical tools for separation and identified composition of the mixture. It use gas as the carrier of the liquid sample through a stationary phase.
Gas-liquid chromatography (GLC), or simply gas chromatography (GC), is a type of chromatography in which the mobile phase is a carrier gas, usually an inert gas such as helium or
Gas-liquid chromatography is often just called as gas chromatography. It is used in analytical chemistry for separating and analyzing compounds that can be vaporized without decomposition
( ′lik·wəd ′krō·mə′täg·rə·fē ) (analytical chemistry) A form of chromatography employing a liquid as the
Explore this Topic
Gas chromatography or specifically gas and liquid chromatography involves a sample being vaporised and injected onto the head of the chromatographic column. The ...
In paper chromatography, a test sample is dotted onto a piece of paper, placed in a liquid that mobilizes its components separately and inspected to determine ...
Chromatography is defined as the method of dividing and analysing the contents of gas or liquid mixtures through selective adsorption. It is also defined as any ...
About -  Privacy -  Careers -  Ask Blog -  Mobile -  Help -  Feedback  -  Sitemap  © 2014 Ask.com