How to Calculate Serial Dilutions?

Answer

A simple dilution is easy to understand. If you've made soup, you know you use one can of the concentrate and one can of water. The concentrate is 1 and the total outcome is 2, giving a dilution ratio of 1:2.&amp;amp;#x0D;
If you were then to take one can of the diluted soup and place it in a separate dish with one can of water, you would have a serial dilution, with a ratio of 1:4. Excellent explanation in further detail is available here: abacus.bates.edu
Reference:
1 Additional Answer
To calculate serial dilutions you will need two test tubes, tube A filled with 10mL of the solution and tube B with 9mL of a buffer. Transfer 1mL from tube A to tube B and mix. The contents in tube B are 10 times more dilute than in tube A. Fill a tube C with 9mL of buffer. Transfer 1mL from tube B to tube C and mix. Now the solution in tube C is 10 times more dilute that tube B and 100 times more dilute than tube A. You can now calculate the total dilution in a serial dilution. The results can be generalized by saying that the total dilution is equal to Dilution 1 times Dilution 2 times Dilution 3 times Dilution N, where the Dilution i is the dilution ratio of the ith dilution.
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