What Is the Mechanism of Staining in Fungi by Lactophenol Cottonblue?

Answer

By staining fungi with lactophenol a microbiologist can clearly see it under the microscope. The cotton blue lactophenol is the most common method of staining fungi. The dye will penetrate the hyphae and reproductive cells in the fungi.
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Phenol will kill any live organisms; lactic acid which preserves fungal structures, and cotton blue which stains the chitin in the fungal cell walls.
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The compound penetrates the hyphae and reproductive cells of the fungi. Often, water-soluble dyes are repelled by the chitinous cell walls of the hyphae. Source(s): Biology instructor
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1. Mix the specimen whether a skin scraping, fluid exudate or tissue with two drops of the 10% KOH on a clean slide. 2. Then add 2 drops of the lactophenol cotton blue. 3. Gently
http://www.drugs.com/vet/jorvet-j-326b-lactophenol...
Hello Salina, Because the gram stain only works with organisms that have cell walls of peptidoglycan, i.e. bacteria only. Plant or fungal cell walls consist of other substances (mainly
http://en.allexperts.com/q/Biology-664/microbiolog...
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A preparation of phenol, lactic acid, glycerin, distilled water, and cotton blue dye, used to stain fungi in wet preparations. ...
It seems like you are referring to staining. The stain is added to specimen of bacteria to check what kind of fungi or bacteria it is. There are different kind ...
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