What Is a Sucrose Gradient?

Answer

A sucrose gradient is used most often to separate biological cellular components by their mass. Several densities of sucrose solution are prepared and carefully layered like a cake in a centrifuge tube, forming a decreasing density gradient from top to bottom. Cellular components are then placed in the tube and spun. Components with greater mass travel to the bottom of the tube, and lighter mass remain near the top.
Q&A Related to "What Is a Sucrose Gradient?"
The National Institute for Medical Research in London reported that alkaline sucrose gradients were used in early research to investigate how DNA synthesis works in certain cultures
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( ′den·səd·ē ¦grād·ē·ənt sen′trif·ə′gā·shən ) (analytical chemistry) Separation
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Cellular constituents have different densities, and discontinuous gradients of sucrose present boundaries where denser components (mitochondria) will pass through, while less dense
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Differential centrifugation. : The solution (eg sucrose) is uniform throughout the test tube. You separate organelles based on their size and density. Density gradient centrifugation
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Differential centrifugation is a technique commonly used by biochemists. Tissue, such as liver, is homogenized at 32°F (0°C) in a sucrose solution that ...
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