What Is the Most Critical Step in Gram Staining?

Answer

The most critical step in gram staining is heat fixation. Fixation is a chemical procedure by which organic materials are conserved from deterioration, thereby preventing autolysis or putrefaction. Fixation dismisses any continuing biochemical reactions, and may also intensify the mechanical strength or stability of the treated tissues.
Q&A Related to "What Is the Most Critical Step in Gram Staining..."
The most critical step of gram staining is the decolorization step as crystal violet stain will be removed from both G+ve & G-ve cells if the decolorizing agent(e.g alchohol )
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Crystal violet, a primary stain (one that imparts its color to all cells), is applied to a heat-fixed smear. After a short time the crystal violet is washed off, and the smear is
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I think its the application of the mordent which in most cases is iodine. The purpose of the mordent is to bind the primary stain to the specimen. If you leave the mordent on too
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Gram stain is used to stain bacteria. This is a differential
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