Why Is It Necessary to Heat Fix Bacterial Smears?

Answer

It is necessary to heat fix bacterial smears prior to staining so that they will accept the stain. Without being heated first, the stain will slide off the surface of the bacteria. It is only necessary in order to kill the bacteria and to help hold them in place on the slide.
Q&A Related to "Why Is It Necessary to Heat Fix Bacterial Smears"
To prevent the bacteria from washing off during staining, to kill the bacteria, etc.
http://wiki.answers.com/Q/Why_is_it_necessary_to_h...
Flaming the loop and test-tube mouth is called 'aseptic technique' and is to prevent contamination. To 'fix' the sample to the slide so it doesn't wash off during the staining process
http://answers.yahoo.com/question/index?qid=200910...
It causes bacteria to adhere to a slide - stained
http://www.chacha.com/question/why-does-a-bacteria...
My professor said it was to help them stick to the slide, so they are not easily washed off.
http://uk.answers.yahoo.com/question/index?qid=201...
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