Topic: API 20e Test
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Answers to Common Questions
Why do you need a humid environment for the API 20E test?
The API 20e has dry reagents in the tubes. Adding the sample adds the initial moisture, but it must be kept moist during incubation for the reactions to fully develop. Some samples may take up to 48 hours at 37 degrees C. Read More »
Source: http://wiki.answers.com/Q/Why_do_you_need_a_humid_environment_for...
What is API 20E?
A commerically available kit used for the identification of Enterobacteriaceae and some other gram-negative bacteria. Read More »
Source: http://www.answers.com/topic/api-20e
Why is an oxidase test performed on a culture before using API 20...
API 20E and Enterotube II are for the identification of oxidase- negative bacteria. If the oxidase test is positive, these two methods of identification would be useless. Read More »
Source: http://wiki.answers.com/Q/Why_is_an_oxidase_test_performed_on_a_c...
More Common Questions
Answers to Other Common Questions
Analytical Profile Index 20 enterobacteria
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Source: http://wiki.answers.com/Q/What_does_abbreviation_API_20E_Stand_fo...
Providing a moist condition for bateria
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Source: http://wiki.answers.com/Q/What_is_the_purpose_of_water_in_the_tra...
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Source: http://wiki.answers.com/Q/What_are_the_results_for_Api_20E_system...
The API 20E System is a miniatuized version of conventional tsts that is used for the identification of members of the family Enterobacteriaceae and other gram-negative bacteria. This system utilizes a plastic strip with 20 separated compar...
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Source: http://wiki.answers.com/Q/How_does_an_API_test_work
API 675 says that the metering pump has to be tested at 1.5 times the MAWP (Maximum Allowable Working pressure) but not less than 20psig (API 675 4.3.2.1)
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Source: http://wiki.answers.com/Q/What_is_API_675_hydrostatic_test
Each cupule is inoculated with a saline suspension of a pure bacterial culture, rehydrating the dried reagent in each tube. Some of the tubes are to be completely filled (tests CIT, VP and GEL), whereas others are topped off with mineral oi...
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Source: http://www.suite101.com/content/api20e-bacterial-id-test-strip-a5...
Disadvantages of using API (20E) strips: (1) Approximately 3%-10% misidentification rate. (2) More sophisticated tests are often necessary to narrow identification to particular species. (3) It is necessary to kill the individuals that are ...
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Source: http://answers.yahoo.com/question/index?qid=20090216033317AA9N0kD