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Acid Fast Stain

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Answers to Common Questions
( ′as·əd ′fast ′stān ) (microbiology) A differential stain used in identifying species of Mycobacterium and one species of Nocardia.
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The Acid-fast stain is specifically used to identify bacteria that possess a waxy lipid within the structure of their cell walls. Due to the presence of this lipid, water-based stains, such as the Gram stain, so not work well on Acid-fast o...
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Your health care provider will collect a sample of blood, urine, stool, sputum, bone marrow, or tissue, depending on the location of the suspected infection. The sample is then sent to a laboratory, where a small amount is placed on a glass...
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Answers to Other Common Questions
Gram stains use dyes withpositive charges that are attracted by negetively charged cell walls. Acid fast stains are usually negativley charged, so it would depend on what you were staining. A gram stain may not be adequate if you are staini...
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The heat is the mordant for an acid-fast stain
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First - The purpose of staining (in general) is to enable one to see cells microscopically by adding color to the cell. This is due to the fact that unstained bacteria are transparent and are difficult to observe. Acid fast stain is a diffe...
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to turn the non-acid cells a different color than the acid-fast cells
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In an acid-fast stain, positive acid-fast bacterium will appear red because they possess a lipid mycolic acid on their membrane and will retain heated carbolfuchsin. Negative acid-fast bacterium will appear blue (stained by methylene blue)....
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The Ziehl-Neelsen stain, also known as the acid-fast stain, was first described by two German doctors; Franz Ziehl (1859 to 1926), a bacteriologist and Friedrich Neelsen (1854 to 1894), a pathologist. It is a special bacteriological stain u...
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both are the type of differential staining and are done in case of microbes in endospore staining malachite green and in acid fast carbol fuschin is used
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