Topic: Biuret Assay
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What the role of potassium iodide in the biuret assay?
I believe it is potassium hydroxide, not potassium iodide. The Biuret reagent is made of potassium hydroxide (KOH) and copper (II) sulfate (CuSO4), together with potassium sodium tartrate (KNaC4H4O6·4H2O). The blue reagent turns violet in t... Read More »
Source: http://answers.yahoo.com/question/index?qid=20080401214155AAoGyfB
What is the principle of biuret method in protein assay and its p...
Proteins are colourless by themselves...Biuret reagents contains copper ions which will complex with the amide gropus or peptide bonds in the proteins to create a blue colour that will be measures using a colorimeter or a spectroscope... th... Read More »
Source: http://answers.yahoo.com/question/index?qid=20070309180027AAC3Sqd
How do lipids, bilirubin, hemoglobin and ammonia interfere with b...
Read that article http://pdf.usaid.gov/pdf_docs/PNADF783.p… Read More »
Source: http://answers.yahoo.com/question/index?qid=20080902044240AAoT62x
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Biuret Assay
