Topic: Biuret Reagent
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Answers to Common Questions
What is the composition of biuret's reagent?
The cupric ion(2+) tends to oxidize the peptide backbone, and produces Cu+. The reaction appears to take place under alkaline conditions at an increased rate. Maybe adding the NaOH first, would probably affect the protein in such a way that... Read More »
Source: http://answers.yahoo.com/question/index?qid=20090516203841AAVk8ul
How Biuret's Reagent Works?
in the biuret test, u try t identify th presence of peptide bonds. can't remember in details what reacts but i know that that's what is identified Read More »
Source: http://answers.yahoo.com/question/index?qid=20071029135245AA71GMM
How does biuret's reagent work in protein testing?
The reagent used in the Biuret Test is a solution of copper sulfate (CuSO4) and potassium hydroxide (KOH). The KOH is there to raise the pH of the solution to alkaline levels; the crucial component is the copper (II) ion from the CuSO4. Whe... Read More »
Source: http://wiki.answers.com/Q/How_does_biuret's_reagent_work_in_prote...
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Biuret Reagent
More Common Questions
Answers to Other Common Questions
When biuret reagent is added to egg white (protein), the solution turns lilac or purple.
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Source: http://wiki.answers.com/Q/What_is_the_reaction_between_biuret_rea...
Copper sulphate gives biuret reagent it's blue colour.
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Source: http://wiki.answers.com/Q/What_gives_the_blue_color_to_biuret_rea...
pinkish-purple
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Source: http://wiki.answers.com/Q/What_color_does_biuret_reagent_turn_whe...
Because skin is a protein and Biuret's reagent turns proteins purple. Therefore, if you get any on your skin, you will be a purple smurf
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Source: http://wiki.answers.com/Q/Why_would_Biuret_reagent_turn_skin_brow...
Biuret test works for any compound containing two or more of these groups: CO-NH, CO-NH2, CH2-NH2, CNH-NH2, CS-NH2. Amino acids only have one.
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Source: http://wiki.answers.com/Q/Why_does_Biurets_reagent_react_with_pro...
Biuret Reagent: Add, with stirring, 300 mL of 10% (w/v) NaOH to 500 mL of a solution containing 0.3% copper sulfate pentahydrate and 1.2% sodium potassium tartrate, then dilute to one liter. The reagent is stable for a few months but not a ...
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Source: http://answers.yahoo.com/question/index?qid=20111120085934AAQy5xb
