Topic: Bradford Assay
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What are the advantages and disadvantages of Bradford protein ass...
Advantages: Compatible with most buffers, reagents and preparations. One step procedure that is sensitive and accurate. Disadvantages: Non linear correlation over large range of protein concentrations. The dye binds to surface of cuvettes s... Read More »
Source: http://wiki.answers.com/Q/Advantages_and_disadvantages_Bicinchoni...
What is the chromophore in the Bradford protein assay?
I think coomassie blue Read More »
Source: http://answers.yahoo.com/question/index?qid=20080409161407AA7vR1p
How can detergents interfere with bradford assay?
the detergents interupt the cross-link hygarious bonds betwwen the molecule chains making up pitahgriotic(-8,-9) polymers Read More »
Source: http://uk.answers.yahoo.com/question/index?qid=20081005171119AABu...
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Bradford Assay
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Bradford protein assay is based on the interaction of Coomassie blue with basic and aromatic amino acids - a complex is made resulting in the shift of the absorbance spectrum of Coomassie blue. Detergents (like SDS) will alter the equilibri...
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Source: http://answers.yahoo.com/question/index?qid=20070907081411AAFM1ee
You need to have a reference graph of concentrations and their absorbances along with a best fit line through the points. Once this is created, you can simply draw a line from the unknown absorbance value across to the best-fit line and the...
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Source: http://answers.yahoo.com/question/index?qid=20100102105336AA1ubcU
Beer's Law breaks down at high concentrations. Dilute samples give a reaction on the linear portion of the curve. One of the disadvantages of the assay is that it has only a fifty- to one-hundred-fold working range.
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Source: http://answers.yahoo.com/question/index?qid=20090706122529AAvA3zR
Use this formula to compute for the concentration: concentration = Absorbance (at wavelength used) divided by absorbance coefficient Source(s): http://www.ruf.rice.edu/~bioslabs/method…
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Source: http://answers.yahoo.com/question/index?qid=20081020104352AA9t2yf
The Bradford Assay is a colorimetric assay for measuring total protein concentration in a given solution. Also known as the Bradford protein assay, it involves the binding of the dye Coomassie Brilliant Blue to protein in an acidic solution...
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Source: http://www.histosoft.com/services/background/ba/baback.html
It is to determine the amount of protein in a sample. It relies on the indicator changing color when it binds to alanine in the protein. If the protein in the sample has very little alanine, it will under-estimate the amount of protein.
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Source: http://au.answers.yahoo.com/question/index?qid=20070828054601AAMT...
