Topic: Chocolate Agar
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How to incubate chocolate agar?
have to incubate at low oxygen content Read More »
Source: http://wiki.answers.com/Q/How_to_incubate_chocolate_agar
What is the reaction of chocolate agar
Bacterial culture of H. influenzae is performed on agar plates, pre... ...MORE... Read More »
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What organisms cannot grow on chocolate agar?
Answer Most pathogenic organisms will grown on chocolate agar, it depends mostly on the incubation conditions i.e. temperature, presence/absence of oxygen etc. However there are some fastidious organisms that require certain growth factors ... Read More »
Source: http://wiki.answers.com/Q/What_organisms_cannot_grow_on_chocolate...
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Chocolate Agar
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Preparation of Chocolate agar: 1. 2L distilled water + 144 gm GC agar powder 2. Dissolve fully with heating till boiling 3. Autoclave 4. Cool till 45 C degree 5. Add 5% defibrinated sheep blood 6. Heat SLOWLY and evenly to 65 C degree 7. Co...
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Source: http://wiki.answers.com/Q/When_do_you_use_chocolate_agar
If you add the blood to the media before it is cooled ie if the media is hot, you will make chocolate agar plates instead of blood agar plates.
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Source: http://wiki.answers.com/Q/How_do_you_turn_blood_agar_plates_into_...
Differing microbes require differing enviroments for growth. In order to completely assess the sample for microbes it is necessary to plate it in several enviroments so as not to miss anything. A microbe may grow very well in blood agar but...
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Chocolate Agar with Bacitracin is recommended for use in the isolation and cultivation of Haemophilus species from respiratory specimens. Source(s): wikipedia
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Source: http://answers.yahoo.com/question/index?qid=20071028032256AALnguB
Chocolate agar is actually hemolyzed blood with some special nutrients added. Note that many Neisseria spp. will grow on blood agar and do not require chocolate agar. For chocolate agar, hemolyzing the red cells releases hemin and there are...
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Source: http://answers.yahoo.com/question/index?qid=20081124072216AAdnkGd
Blood agar and Chocolate agar are very similar. The biggest difference is that in choc agar, the preparation includes adding the 5% sheep blood at a higher temperature(between 75-80C) causing the Red Blood Cells to lyse and thus creating th...
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Source: http://answers.yahoo.com/question/index?qid=20070517203056AAHVbYv