Topic: DNA Analysis Lab Restriction Digest
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Answers to Common Questions
What is the price of a DNA analysis lab?
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Source: http://wiki.answers.com/Q/What_is_the_price_of_a_DNA_analysis_lab
How do you purify impure DNA after restriction digestion?
You can do a tedious phenol-chloroform extraction, or do it the easy way: QIAquick PCR purification kit where you bind the DNA to a column and elute it off in water or TE. You will lose some of your DNA though so keep this in mind. Read More »
Source: http://wiki.answers.com/Q/How_do_you_purify_impure_DNA_after_rest...
What happens when DNA is digested by a restriction enzyme?
Restriction enzymes recognize specific sequences in DNA and... Read More »
Source: http://www.chacha.com/question/what-happens-when-dna-is-digested-...
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Answers to Other Common Questions
We generally add milliQ (DNase free) water. For digestion water is added usually. It provides right Hydrogen bonds between enzyme amino acid residues and bases present in the restriction sites. Utpal Roy
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Source: http://wiki.answers.com/Q/Why_you_add_distilled_water_in_restrict...
A restriction enzyme recognizes and ...
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Source: http://www.chacha.com/question/how-are-restriction-enzymes-used-t...
Plasmid DNA is typically supercoiled, which makes it run faster on a gel. When you digest the plasmid with a restriction enzyme, it linearizes the DNA. When there is a single cut site, you now have a very long piece of DNA that must migrate...
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Source: http://answers.yahoo.com/question/index?qid=20101126095654AA9wfVl
I'm guessing you want to transform the genomic DNA with the plasmid DNA? If so, it's because you want the cuts to be mae in the same place, so both sequences are compatible.
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Source: http://answers.yahoo.com/question/index?qid=20120325161924AAF5DNP
1.DNA methylation 2.The restriction enzyme DNA target sequence is absent.
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Source: http://answers.yahoo.com/question/index?qid=20091122170857AACvLWy
If PCR is an option, go with that.
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Source: http://answers.yahoo.com/question/index?qid=20090514110136AAEUwKm
Isnt only 1 enzyme supposed to be used? So that it would cut the DNA at the same restriction sites and similar fragments are produced for better comparison? Source(s): me
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Source: http://answers.yahoo.com/question/index?qid=20090417120416AAXCUFP
