Topic: Description of a Restriction Digest
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What is the description of the role of the esophagus in digestion...
Simple carbohydrates are broken down here, but it is mainly for transportation of the bolus to the stomach. Read More »
Source: http://wiki.answers.com/Q/What_is_the_description_of_the_role_of_...
What is a Brief description of the digestive system?
Teeth tare apart the food saliva is added. Then, the muscles pushes food to the stomach, the stomach mixes up the food acid is added. In the small intestines, nutrients are taken out sent to the blood, in the large intestines water is taken... Read More »
Source: http://wiki.answers.com/Q/What_is_a_Brief_description_of_the_dige...
What is restriction digest?
A unique family of DNA fragments produced by digestion of a given DNA molecule by a particular restriction enzyme; usually visualized by agarose gel electrophoresis and ethidium bromide or other fluorescent procedures. Read More »
Source: http://www.answers.com/topic/restriction-digest
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Description of a Restriction Digest
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I believe that it is used to stabilize the pH levels so the enzyme activity isn't effected.
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Source: http://wiki.answers.com/Q/What_is_the_purpose_for_adding_restrict...
You can do a tedious phenol-chloroform extraction, or do it the easy way: QIAquick PCR purification kit where you bind the DNA to a column and elute it off in water or TE. You will lose some of your DNA though so keep this in mind.
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Source: http://wiki.answers.com/Q/How_do_you_purify_impure_DNA_after_rest...
We generally add milliQ (DNase free) water. For digestion water is added usually. It provides right Hydrogen bonds between enzyme amino acid residues and bases present in the restriction sites. Utpal Roy
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Source: http://wiki.answers.com/Q/Why_you_add_distilled_water_in_restrict...
Restriction enzymes recognize specific sequences in DNA and...
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Source: http://www.chacha.com/question/what-happens-when-dna-is-digested-...
Restriction Digestion is the process of cutting DNA molecules into smaller pieces with special enzymes called Restriction Endonucleases (sometimes just called Restriction Enzymes or RE's). These special enzymes recognize specific sequences ...
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Source: http://answers.yahoo.com/question/index?qid=20110125181747AAxXmBU
http://www.fermentas.com/catalog/kits/ge... This site describes the whole process of the kit.
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Source: http://answers.yahoo.com/question/index?qid=20090610161841AAzD9u1
