Topic: Effects of Triton X 100
Answers to Common Questions
How does Triton X 100 effect cells?
Triton-X100 permeabilizes cell membranes, and allows reagents access to both the cytosol and nuclear material. Triton-X100 is often used to stain fixed cells with antibodies, especially for BrdU or DAPI type staining. Read More »
Source: http://wiki.answers.com/Q/How_does_Triton_X_100_effect_cells
How to make 0.1 percent Triton X-100?
0.1 % Triton X-100 is a 1 in 1000 dilution of 100% Triton X-100 (available for purchase). For example, add 1ml 100% Trition to 999ml water to yield 0.1% Triton. I usually keep a stock of 10% triton on hand to make various buffers. Read More »
Source: http://wiki.answers.com/Q/How_to_make_0.1_percent_triton_X-100
What is the difference between Triton X100 and CHAPS detergent?
There are multiple differences. Triton X-100 is non-ionic . It has both hydrophilic and hydrophobic regions, but no net charges. CHAPS is zwiterionic. It has hydrophobic regions but also a head group with a negative charge (in normal saline... Read More »
Source: http://wiki.answers.com/Q/What_is_the_difference_between_Triton_X...
Answers to Other Common Questions
Low concentrations don't really affect LDH activity too much. Cytotoxicity assays that measure release of LDH as a marker for cell death use Triton X-100 to lyse the control cells (to show 100% lysis), so if it diminished activity, you'd ha... Read More »
Source: http://answers.yahoo.com/question/index?qid=20060927212013AAoAxs4
Try coleparmer.com. Read More »
Source: http://www.quora.com/Where-can-I-buy-Triton-X-100
Try www.chemfinder.com for the first one. Read More »
Source: http://answers.yahoo.com/question/index?qid=20070530190249AAgqz7z
Ammonium hydroxide is an aqueous solution of ammonia in water. It is commercially available in different concentrations. Lets say we begin with a concentration of 20 % (w/w) (20 g ammonia in 100 g solution). A 0.05 % (w/w) solution means th... Read More »
Source: http://uk.answers.yahoo.com/question/index?qid=20110720054503AARx...
Use the molecular weight of the glycine from your source. Multiply by 0.05, and use that many grams per liter of end product. Read More »
Source: http://answers.yahoo.com/question/index?qid=20070819190749AAVa0na
The AK-47 (WASR-10) and AKM, with the 7.62 x 39mm cartridge, have an effective range of about 382.764 yards (350 m/1,150 ft). Read More »
Source: http://www.kgbanswers.com/what-is-the-effective-range-in-yards-of...
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