Topic: Emb Agar
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What is EMB agar?
Eosin Methylene Blue Agar (EMB) was developed by Holt-Harris and Teague.1 This formula contains lactose and sucrose with two indicator dyes, Eosin Y and Methylene Blue. The use of Eosin Y and Methylene Blue as indicators produced sharp and ... Read More »
Source: http://wiki.answers.com/Q/Is_EMB_selective_or_differntial_media
Why Ecoli produces metallic sheen in EMB agar?
The metallic sheen is due to the large amounts of acid that E.coli produces on the EMB agar. The acid causes an amide bond to form between the two dyes (eosin and methylene blue) giving rise to the sheen. Read More »
Source: http://wiki.answers.com/Q/Why_Ecoli_produces_metallic_sheen_in_EM...
What does the EMB agar plate select for?
EMB plate testing is used to look for bacteria with lactose fermentation. + test results show dark purple, blackish or metalic green bacteria colony. On EMB plate, following results showed with 4 different bacteria. E. aerogenes: Purple col... Read More »
Source: http://wiki.answers.com/Q/What_is_the_basis_of_selectivity_for_EM...
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Emb Agar
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Answers to Other Common Questions
EMB Agar is: Differential due to: the presence of two stains which react to varying PH from the fermentation of Lactose Selective due to: the presence of Eosin Y and Methylene Blue, which inhibit the growth of gram positive bacteria.
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Source: http://answers.yahoo.com/question/index?qid=20111207113801AAF2EfS
Gram + bacteria do NOT grow on EMB agar, EMB agar inhibits their growth, and is selective for Gram -
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Source: http://wiki.answers.com/Q/Why_does_gram_positive_bacteria_grow_on...
Escherichia coli-Salmonella-Shigella-Enterobacter
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Source: http://wiki.answers.com/Q/Which_bacteria_gives_dark_puple_colour_...
eosin Y and methylene blue, besides being dyes also inhibit gram-positive bacteria Source(s): http://www.bd.com/ds/technicalCenter/ins…
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Source: http://answers.yahoo.com/question/index?qid=20100219220817AAyPglY
It is mainly because only gram-negative bacteria grow on EMB agar. (Gram-positive bacteria are inhibited by the dyes eosin and methylene blue added to the agar.) EMB is good for growing gram-negative bacteria that causes enteric or urinary ...
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Source: http://answers.yahoo.com/question/index?qid=20071029154352AAQ4IbI
Many type of bacteria can growth on this agar. EMB is abbr of Eosin Methylene Blue agar and the main role of this agar is to isolate E. coli (I am not confirms it is for isolate only one strain or not?). When E. coli growth on this agar, it...
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Source: http://answers.yahoo.com/question/index?qid=20071213202357AAX9das
