Topic: Ethidium Bromide
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Why ethidium bromide is carcinogenic?
The purpose of ethidium bromideis as an intercalating agent commonly used as a fluorescent tag (nucleic acid stain) in molecular biology laboratories for techniques such as agarose gel electrophoresis. When exposed to ultraviolet light, it ... Read More »
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What is ethidium bromide?
( e′thid·ē·əm ′brō′mīd ) (organic chemistry) C21H20BrN3 Dark red crystals with a melting point of 238-240°C; used in treating trypanosomiasis in animals and as an inhibitor of deoxyribonucleic and ribonucleic acid synthesis. Also known as h... Read More »
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What is the role of Cscl and ethidium bromide in plasmid purifica...
The CsCl forms a gradient and the molecules migrate according to their density until they float at their individual isopycnic points (the point in the gradient that equals the buoyant density of the molecule). However, plasmid DNA and conta... Read More »
Source: http://wiki.answers.com/Q/What_is_the_role_of_Cscl_and_ethidium_b...
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Ethidium Bromide
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Answers to Other Common Questions
EtBr interculates into DNA and when exposed to UV light, it causeses DNA to nick and therefore uncoil
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Source: http://wiki.answers.com/Q/What_is_the_function_of_ethidium_bromid...
Ethidium bromide binds with DNA and slips in between its hydrophobic base pairs and stretches the DNA fragment, removing water molecules from the ethidium cation. The result of this dehydrogenation is an increase in fluorescence of the ethi...
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Source: http://wiki.answers.com/Q/How_does_ethidium_bromide_work
Ethidium bromide is a chemical that is able to intercalate (fit between the bases) between the bases of double stranded DNA strands
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Source: http://wiki.answers.com/Q/What_does_ethidium_bromide_stain_in_a_c...
ethidium bromide is helping to visualize the dna under uv light.if the dna is presnt in the gel, the ethi.bro will bind with it & it will emitt fluoresence.some other fluorescent dyes also available, but they r not showing good results,so e...
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Source: http://wiki.answers.com/Q/Why_is_ethidium_bromide_added_to_the_ag...
Ethidium bromide is a fluorescent dye used for staining nucleic acids. Ethidium bromide is a known mutagen, handle carefully.
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Source: http://www.chacha.com/question/what-is-the-purpose-of-ethidium-br...
This fluorescent dye,contains a tricyclyic planar group and intercalates between stacked base pairs of nucleotides.
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Source: http://www.chacha.com/question/what-are-the-all-the-functional-gr...
