Topic: Hopkins Cole
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What is the composition of Hopkins cole reagent?
3.0 g Mg powder or thin ribbon cut in small pieces, cover with distilled water and cool in an ice bath. Slowly add with stirring 75 mL cold, saturated oxalic acid solution (about 8 g oxalic acid in 75 mL water). Filter, and 7.5 mL glacial a... Read More »
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What is the Hopkins-Cole test?
Answer The hopkins-cole test is used to determine the presence of the amino acid tryptophan. Tryptophan has an indole nucleus which is responsible for the violet ring found at the junction between the two layers. Read More »
Source: http://wiki.answers.com/Q/What_is_the_Hopkins-Cole_test
What is Hopkins-Cole reaction?
( ′häp·kənz′kōl rē′ak·shən ) (analytical chemistry) The appearance of a violet ring when concentrated sulfuric acid is added to a mixture that includes a protein and glyoxylic acid; however, gelatin and zein do not show the reaction. Read More »
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This test only works for proteins containing tryptophan, which is the only amino acid which contains and indole ring (a 5 membered ring, including one nitrogen, in the ring, and one double bond) (much the same as pyridine, but with a double...
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Millon's test is given by any compound containing a phenolic hydroxy group. www.chemistry.mcmaster.ca/~chem2o6/lab… - 24k - The hopkins-cole test is used to determine the presence of the amino acid tryptophan. http://wiki.answers.com/Q/What...
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The indole group of tryptophan will react with glyoxylic acid in the presence of concentrated H2SO4 to give a purple color. http://209.85.175.132/search?q=cache:aft… For more details, please visit : http://jdr.iadrjournals.org/cgi/reprint/…...
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