Topic: How Does a UV Vis Spectrometer Work
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How to Zero a UV/Vis Spectrometer
Having a proper zero on an ultraviolet-visible (UV-Vis) spectrometer is vital to making accurate measurements. The process is normally very straightforward and uncomplicated if you have past experience with a UV-Vis. "Zeroing" a UV-Vis prov... Read More »
Source: http://www.ehow.com/how_5901509_zero-uv_vis-spectrometer.html?ref...
What is a UV/Vis Spectrometer?
s uv/vis spectrophotometer is an instrument with a dispersion device ( a unit that can break white light into the constituent wavelengths ( usually a diffraction grating or on occasion, a prism. ( although prisms use refraction rather than ... Read More »
Source: http://answers.yahoo.com/question/index?qid=20080628163732AAIG6y6
How does UV-vis instrument ( double beam spectrometer)works?
The principle of a double beam spectrometer is that you can simultaneously measure the sample and reference cuvettes. If you block the sample beam, the spectrometer will think that no light is coming through, resulting in ridiculously high ... Read More »
Source: http://answers.yahoo.com/question/index?qid=20070313101501AAyXWsM
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Answers to Other Common Questions
This is a really good question as it got me thinking.... my answer/guess is...... aspirin = acetylsalicyclic acid is not very soluble in water. soluble aspirin in shops are sodium acetylsalicylate which then reforms into aspirin (acetylsali...
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Source: http://uk.answers.yahoo.com/question/index?qid=20061018133938AAJ3...
It is a spectra that shows how transparent a material can be. in scientific terms, it is incidence of electromagnetic radiation (from the UV, Visible, Infrared wavelength regions) from a source to a material, and there is a detector which r...
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Source: http://wiki.answers.com/Q/What_is_UV-VIS-IR_transmission_spectra
The Beer-Lambert law Absorbance = (extinction coefficent)(pathlength of light)(concentration) allows you to measure the absorbance of sample in a UV spec, and change the rate from absorbance units / time to change in concentration / time. t...
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Source: http://wiki.answers.com/Q/What_is_the_relation_of_Beers_Lambert's...
The answer is very complicated. There is no short answer. Courses in chemistry would be needed to explain. In general, the readout gives you a relative absorption of a wavelength of light passing thru a special sample of a chemical. By usin...
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Source: http://wiki.answers.com/Q/How_do_you_analyse_a_UV-vis_readout
The difference between the VIS & UV is the wavelength which is 3201100nm (visible range) for the VIS while it is 1901100nm in UV
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Source: http://www.chacha.com/question/what-is-the-difference-between-a-u...
That means your blank has a higher absorption at that particular wavelength than the sample. This really shouldnt happen assuming your blank is water.
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Source: http://wiki.answers.com/Q/What_does_it_mean_if_one_found_negative...
it happened to me too in one of my inorganic labs i think it is external light getting into the spectrophotometer Note: There are comments associated with this question. See the discussion page to add to the conversation.
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Source: http://wiki.answers.com/Q/What_does_it_mean_if_you_get_a_negative...
