Topic: Lowry Protein Assay
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Answers to Common Questions
What are the advantages and disadvantages of Bradford protein ass...
Advantages: Compatible with most buffers, reagents and preparations. One step procedure that is sensitive and accurate. Disadvantages: Non linear correlation over large range of protein concentrations. The dye binds to surface of cuvettes s... Read More »
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How long the blue colour is stable in protein estimation by lowry...
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What methods of assay are are there for a protein?
Quantitative assasy if you have a pure sample : Go for Lowry's method of assay if the sample is contaminated by some ions or other chemicals : then go for Bradford's method of assay beasuce it is not affected by contaminants , but the limit... Read More »
Source: http://answers.yahoo.com/question/index?qid=20070918051054AADSM3n
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Lowry Protein Assay
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Answers to Other Common Questions
EMSA does not measure if protein bends to DNA. It does measure what proteins bind to a specific region of DNA (usually a promoter region). You can use a supershift to determine exactly what protein is binding to the specific DNA region.
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Source: http://wiki.answers.com/Q/What+is+electromobility+shift+assy
I think coomassie blue
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If you are just trying to figure out whether a particular protein is present within a complex mixture and/or whether it is increased with treatment, you can do a western blot whether or not the protein is an enzyme. In this process, the pro...
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Source: http://answers.yahoo.com/question/index?qid=20070916124347AAiP0uR
label the known protein with a radioactive isotope, or better yet, maybe you could label the protein with a flourescent dye (dont know how, its beyond my expertise) and where ever the protein binds on the receptive cell may be what you're l...
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Source: http://answers.yahoo.com/question/index?qid=1006031213538
Different proteins absorb maximally at different wavelengths. So, depending on the wavelength used in the spectrophotometer, you could use the spectrophotometer to determine the concentration of a specific protein in a solution or to identi...
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Source: http://answers.yahoo.com/question/index?qid=20120221135906AACJQCo
You would want to do a tenfold dilution pattern, meaning that you remove x amount of the substance you want to dilute, and add x amount of the solvent or whatever you are diluting with. You would then repeat this five times going from 20-->...
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Source: http://answers.yahoo.com/question/index?qid=20100331102107AA3kQLO
