Topic: Northern Blot
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How to Interpret the Results of Northern Blots
Northern blots are mostly a tool in RNA (ribonucleic acid) research. The RNA is extracted from the specimen and run on a gel for electrophoresis that is then transferred to a filter membrane. The type of RNA that is used in Northern blots i... Read More »
Source: http://www.ehow.com/how_7360902_interpret-results-northern-blots....
What is Northern blot?
A technique for separating RNA molecules by electrophoresis and then identifying a target fragment with a DNA probe. Read More »
Source: http://www.answers.com/topic/northern-blot
What is Northern blot analysis?
n. A technique for identifying specific sequences of RNA in which RNA molecules are separated by electrophoresis, transferred to nitrocellulose, and identified with a suitable probe. [Playful coinage after SOUTHERN BLOT ANALYSIS.] Read More »
Source: http://www.answers.com/topic/northern-blot-analysis
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Northern Blot
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The transfer buffer used for the blotting usually contains formamide because it lowers the annealing temperature of the probe-RNA interaction, thus preventing RNA degradation by high temperatures.And also The RNA samples are most commonly s...
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Source: http://wiki.answers.com/Q/Why_use_formaldehyde_in_northern_blotti...
Used for identification of RNA blotted and immobilized on a membrane...
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Source: http://www.chacha.com/question/what-does-a-northern-blot-hybridiz...
A Southern blot is used for DNA; DNA is separated by length with gel electrophoresi...
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Source: http://www.chacha.com/question/how-is-northern-blotting-done-in-d...
A Southern blot is a method in molecular biology of enhancing the result of an agarose gel electrophoresis by marking specific DNA sequences. The method is named after its inventor, the British biologist Edwin Southern.[1] This caused other...
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Source: http://answers.yahoo.com/question/index?qid=20070209213111AAVTGfM
why? 1) used to detect particular RNA from a mixture of RNAs. 2) to find tissu-specific amount of paticular RNA. How? samle mixture of RNAs taken and treated with formaldehyde(to prevent hydrogen bonding). then the mixture is transferred to...
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Source: http://answers.yahoo.com/question/index?qid=20081005102802AA10RC2
The same basic principles apply to both. The main difference being that southern blot is for DNA while northern is for RNA. The process involves the separation of pieces of the nucleic acid by size using electrophoresis. Afterwords, the DNA...
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Source: http://answers.yahoo.com/question/index?qid=20090716162147AAIlSR2
