Topic: PCR Process
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What is the the process of PCR
PCR is a process based on the ability of a DNA polymerase enzyme th... ...MORE... Read More »
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What is used to divide the dna molecule in the PCR process?
Typically, PCR consists of a series of 20-40 repeated temp... Read More »
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Why are primers needed in the PCR process?
Because DNA polymerases cannot initiate DNA synthesis of a new strand de novo; they can only extend a preexisting strand. Even during normal DNA replication (S phase of interphase), DNA polymerases only extend preexisting sequences. An RNA ... Read More »
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PCR Process
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It uses a Peltier block to heat and chill the tubes very rapidly. It does it over and over again through the PCR, that's why it's called thermal cycler (thermal = heats and chills, cycler = over and over).
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PCR or Polymerase Chain Reaction mimics DNA replication. PCR starts with a template DNA (or RNA) and generates new copies of DNA by synthesizing using a heat-stable DNA polymerase.Even when starting with an RNA template, after one round of ...
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By using primers complementary to the ends flanking the sequence to be amplified.
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your cells use DNA polymerase similar to Taq polymerase used in PCR to replicate DNA strands. The polymerase reads the nucleotides and makes a new strand. in you cells you are replicating the entire genome for the process of cell division f...
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