Topic: PCR Protocols
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Answers to Common Questions
What is the Roche PCR protocol?
Gives detailed general protocol for PCR, including preparing reaction mixture, cy... Read More »
Source: http://www.chacha.com/question/what-is-the-roche-pcr-protocol
RT-PCR equipment validation protocol template...where can I find?
I would go to the website of the company where it was bought. Most have protocols available. Read More »
Source: http://answers.yahoo.com/question/index?qid=20090206174525AAe4WRx
What is the key to maximizing protocol speed with the High Perfor...
In High Performance PCR, the polymerase, the thermal cycler and the vessel each offer time savings through different mechanisms. Click here for a chart with a more detailed explanation. Read More »
Source: http://www.finnzymes.net/hppcr_faq.html?select2=none
More Common Questions
Answers to Other Common Questions
It will be straightforward! The PCR conditions for our markers are very robust. In order to enable multiplex PCR, we use whenever possible a standard PCR protocol for amplifying all microsatellites. However, in some cases we must adapt the ...
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Source: http://www.ecogenics.ch/e/faq.html
The most common application of real-time PCR in our facility is the study of gene expression in which we measure the relative gene expression levels in test versus control samples. Depending on the purpose of each experiment,we select the t...
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Source: http://www.genomeweb.com/node/920917
This is due to differences in composition between PCR and RT-PCR buffers. QuantiFast PCR Buffers are optimized for fast amplification with shortest possible PCR steps, while QuantiFast RT-PCR Buffers ...
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Source: http://www.qiagen.com/faq/faqlist.aspx?SearchText=&FaqCategoryId=...
A normal qPCR MasterMix (reference RT-QP2X-03, RT-QP2X-03-075+, RT-QP2X-03-15+, RT-QP2X-50+) includes the UNG enzyme. To avoid any degradation of the PCR products after the PCR is completed, the protocol state hold at 50 °C forever, to keep...
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Source: https://secure.eurogentec.com/EGT/Default.aspx?Ctrl=DetailFaq&Id=...
