Topic: Plasmid Vector
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What is plasmid cloning vector?
( ′plaz·mid ′klōn·iŋ ′vek·tər ) (genetics) A plasmid that accepts foreign deoxyribonucleic acid (DNA) and is therefore used in recombinant DNA experiments. Read More »
Source: http://www.answers.com/topic/plasmid-cloning-vector
Why are plasmids used as vectors
Plasmid cloning vectors are engineered to minimize the amount of manipulation of DNA fragment components. Read More »
Source: http://www.chacha.com/question/why-are-plasmids-used-as-vectors
How do you insert a gene into a plasmid vector?
First you either find restriction sites within the gene, or add some to the ends using custom primers. Then you digest the gene with restriction enzymes specific to those sites. You can choose to make them different if the gene's orientatio... Read More »
Source: http://wiki.answers.com/Q/How_do_you_insert_a_gene_into_a_plasmid...
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Plasmid Vector
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A plasmid vector available today is made with a specific host in mind. For example, if you decide to express a gene in a bacteria, there will be plasmids available with features that suit the particular organism that you wish to transform a...
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Source: http://wiki.answers.com/Q/How_are_plasmid_vectors_used_to_transfe...
About 1-5 kb (kilo base) can be inserted in a plasmid vector.
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Source: http://wiki.answers.com/Q/What_is_the_insert_capacity_of_plasmid_...
Plasmids are double-stranded generally circular DNA sequences that are capable of automatically replicating in a host cell. Plasmid vectors minimalistically consist of an origin of replication that allows for semi-independent replication of...
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Source: http://answers.yahoo.com/question/index?qid=20091203175113AApTUNZ
plasmid vector is an extrachromosomal piece of DNA .Its small and circular. It also has unique restriction sites so if you cut the plasmid and gene with same restriction enzyme then you can reanneal the whole thing faster .Mostly for geneti...
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Source: http://answers.yahoo.com/question/index?qid=20090719122648AAi0kAw
A cloning vector is a small piece of DNA into which a...
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Source: http://www.chacha.com/question/what-is-the-relationship-between-t...
A vector is a plasmid (usually) that has been engineered to readily accept foreign DNA via recombination. There is also usually special genes previously inserted which code for something that would allow you to distinguish between the colon...
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Source: http://wiki.answers.com/Q/An+enzyme+used+to+seal+nicks+between+an...
