Topic: Protein Determination Using Biuret Test
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What is a positive test for protein using biuret reagent?
Johncey quintion protein says a positive result involves Pokemon evolving from their first form. Read More »
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What color is a negative biuret test for protein?
A negative test would result in a color that is not violet. Read More »
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How does biuret's reagent work in protein testing?
The reagent used in the Biuret Test is a solution of copper sulfate (CuSO4) and potassium hydroxide (KOH). The KOH is there to raise the pH of the solution to alkaline levels; the crucial component is the copper (II) ion from the CuSO4. Whe... Read More »
Source: http://wiki.answers.com/Q/How_does_biuret's_reagent_work_in_prote...
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Protein Determination Using Biuret Test
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The biuret test is a chemical test used on proteins for detecting t... ...MORE...
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Source: http://www.chacha.com/question/what-is-the-biuret-test-used-for
The bond between the amino group and the carboxyl acid group on adjacent amino acids in a protein is a peptide bond. When the Biuret reagent (1 percent solution of copper sulfate) is added to a solution containing peptide bonds, the solutio...
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To alkaline the medium so that the reaction can take place
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Source: http://wiki.answers.com/Q/What_the_uses_of_sodium_hydroxide_in_th...
i think that all proteins give a posotive biuret test because of the presence of peptide bonds
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during mechanism its 'OH' ion removes a hydrogen ion from nitrogen and therefore is responsible to couple up the molecules.
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Source: http://wiki.answers.com/Q/Why+is+sodium+hydroxide+add+before+addi...
-Absorption Spectroscopy The Protein Peptides absorb some blue (degradation) photons and leaves a purple color. The intensity of the resulting color, determines the amount of Protein.
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Source: http://wiki.answers.com/Q/Why_does_the_biuret_test_make_a_violet_...
