Topic: Purpose of Gram Staining
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What is the purpose of alcohol in gram staining?
The alcohol is a decolorizer. In gram negative organisms, the small amount of peptidoglycan can't hold onto the crystal violet in the presence of the alcohol and so becomes decolorized. The gram positive organisms have a much thicker peptid... Read More »
Source: http://wiki.answers.com/Q/What_is_the_purpose_of_alcohol_in_gram_...
What is the purpose of gram staining the bacteria?
Gram staining is a simple staining test that simply identifies the two main groups of bacteria. Gram positive, and gram negative. Down a microscope, gram pos look like a dark blue/purple colour, and gram neg look red. It is to do with what ... Read More »
Source: http://wiki.answers.com/Q/What_dyes_can_used_for_direct_staining_...
What is the purpose of a decolorizer in the gram stain?
The decolorizer, usually acetone or alcohol, is used to wash the Crystal Violet stain from the Gram Negative cells. From this point Safranin stain is used to stain the Gram Negative cells. The final color for Gram Negative will be a Red/Pin... Read More »
Source: http://wiki.answers.com/Q/What_is_the_purpose_of_a_decolorizer_in...
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Purpose of Gram Staining
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Gram staining is important when trying to look at blood or other liquid under a microscope. Staining allows whatever is in the blood to be stained enough to see and stand out.
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Source: http://answers.ask.com/Health/Diseases/why_is_gram_staining_impor...
Gram staining was created by scientist Hans Gram. It is defined as differentiation between two groups; a positive and/ or negative gram. What factors into the gram staining are chemical and physical agents of the cell wall.You can find more...
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Source: http://answers.ask.com/Health/Diseases/what_is_gram_staining
Gram staining (Gram's method) is a technique used to differentiate bacterial species. Samples of bacteria are stained, then viewed under a microscope to tell if they are gram positive or gram negative.
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to decolorize the color stain?washes away any crystal violet that is not bonded
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Source: http://wiki.answers.com/Q/What_is_the_purpose_of_alcohol_used_in_...
Iodine is added to form the crystal violet-iodine complex so that the dye cannot be removed easily. This "fixes" the dye.
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Source: http://www.chacha.com/question/what-is-the-purpose-of-iodine-in-a...
Gram staining (or Gram's method) is a method of differentiating bacterial species into two large groups (Gram-positive and Gram-negative) based on the chemical and physical properties of their cell walls. The Gram stain is almost always the...
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Source: http://answers.yahoo.com/question/index?qid=20100205102610AANbaUw
