Topic: Site Directed Mutagenesis
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What are the site directed mutagenesis methods?
I would just go to stratagene.com and search their kits. I have used them in the past on a difficult RNA splicing project as an undergraduate. Basically, the RNA folded in such a way that two residues were touching. However, looking at the ... Read More »
Source: http://answers.yahoo.com/question/index?qid=20070826130310AAmxJcl
Why use alanine for site-directed mutagenesis?
Alanine is one of the structurally simpler amino acids. The technique called "alanine scanning" involves systematically altering amino acids in a protein in order to see which is important for structure and function. Eg., aspartate and glut... Read More »
Source: http://answers.yahoo.com/question/index?qid=20080601175436AA3t7CN
How does site directed mutagenesis (using PCR) work; how long doe...
Site-directed mutagenesis is a molecular biology technique in which a mutation is created at a defined site in a DNA molecule, usually a circular molecule known as a plasmid. In general, site-directed mutagenesis requires that the wild-type... Read More »
Source: http://answers.yahoo.com/question/index?qid=20070723083713AARnY5d
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