Topic: Spectrophotometer Experiments
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Answers to Common Questions
What was measured with the spectrophotometer in this experiment?
That other guy was a douche. It measures the color change. That gives the indication of the rate of the light reactions of the photosynthesis in the conditions of the experiment. Read More »
Source: http://wiki.answers.com/Q/What+does+a+spectrophometer+measure
What are the function of NaOH in the experiment of determination ...
NaOH is added to p-nitrophenol to change it into p-nitropenyl, which can give the maximum Absorption. Read More »
Source: http://wiki.answers.com/Q/What_are_the_function_of_NaOH_in_the_ex...
How do you prepare a blank for spectrophotometer experiment?
I have no idea, but since no one else is answering... here's what I remember of this after ...what, 30+ years. I might be wrong. Rinse your cuvette pair with acetone, and allow to air dry fully. Fill both cuvettes with the solvent or buffer... Read More »
Source: http://answers.yahoo.com/question/index?qid=20120227191954AAheWCP
More Common Questions
Answers to Other Common Questions
How it did it was to focus the light and pass it through a narrow slit. This beam was then passed through a prism or onto a diffraction grating and passed to a photo cell where the location and intensity of the separated frequencies can be ...
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Source: http://answers.yahoo.com/question/index?qid=20120327151954AAZHaFn
This is the abosrbance wavelength for the culture. If you set the spectrophtometer during the experiment, then your results will vary. The wavelength will be off and also cells settle to the bottom of the cuvette. good luck.
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Source: http://answers.yahoo.com/question/index?qid=20091029002547AA9nV9N
Biological chemicals absorb light in the UV-Visible range due to resonance of bonds. Basically, 600nm is used because the cells absorb at this wavelength. Thus, when you measure absorbance, you will be indirectly measuring the amount of cel...
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Source: http://answers.yahoo.com/question/index?qid=20081029192831AA9nkD9
More info would be useful, but I think I might know what you're talking about. I'm assuming your solution is purplish/violet and you're measuring absorbance? In that case, you have to measure the absorbance of the complementary color to wha...
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Source: http://answers.yahoo.com/question/index?qid=20080918233425AAftUVS
To optimize sensitivity of the spectrophotometer when you're preparing a curve like that, you should always use the wavelength at which the substance demonstrates maximum absorbence. This is 410 nm for p-nitrophenol. The maximum absorption ...
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Source: http://answers.yahoo.com/question/index?qid=20071201234539AA5c4KA
