Topic: Streak Plate Isolation Method
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Answers to Common Questions
What are the disadvantages of using the streak plate method of is...
In streak plate method we can isolate the different types of bacteria from the given suspension, but we can not count the total number of bacteria present in that suspension Read More »
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How pure cultures can be isolated by using the streak plate metho...
Divide your agar plate into 4 quadrants, A sterile inoculating loop is dipped ito mixed culture, streaked in a pattern over surface of nutrient medium. As pattern transferred, bacteria are rubbed off into the medium. sterilize in between qu... Read More »
Source: http://wiki.answers.com/Q/How_pure_cultures_can_be_isolated_by_us...
Why does the streaking method used to inoculate your plates resul...
Between each set of streaks you sterilize the innoculating loop in the bunsen flame. At the beginning of the next streak you overlap with the end of the one before. The effect of teh technique is to "dilute" the bacteria by gradually spread... Read More »
Source: http://wiki.answers.com/Q/Why+does+streaking+method+use+to+inocul...
More Common Questions
Answers to Other Common Questions
Streak plating allows for the formation of individual colony forming units for isolation by reducing the number of bacterial cells with each streaked quadrant.
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The purpose is to take the mixed sample and isolate the bacteria into pure colonies.
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Source: http://wiki.answers.com/Q/Why+do+the+do+streak-plate+method
Because you flame the loop between successive streakings, each time the bacteria are drawn out there are fewer and fewer individual bacteria, being spread out thinner and thinner. Finally, at the 4th quadrant, the loop is spreading the bact...
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Source: http://answers.yahoo.com/question/index?qid=20090802083622AABqDyf
Streak Plate method cannot be used for the enumeration of the approximate number of bacteria in the given sample. It can used only for obtaining isolated colonies in Pure culture. This is because, In streak plate method, 1. the amount of in...
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Source: http://wiki.answers.com/Q/What_are_the_disadvantages_using_streak...
anaerobic bacteria will not grow
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Robert Koch
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It is much smaller and easier to sterilize during transport. Much more effective instrument.
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Source: http://wiki.answers.com/Q/Why_is_a_needle_used_to_isolate_individ...