Topic: What Are the Characteristics of a Cloning Vector
Answers to Common Questions
What are the typical characteristics of a cloning vector?
Source: http://answers.yahoo.com/question/index?qid=20090104111021AAoH9c4
What is TA cloning vector?
TA Cloning is one of the most popular methods of cloning the amplified PCR product using Taq and other polymerases. These polymerases lack 5'-3' proofreading activity and are capable of adding adenosine triphosphate residue to the 3' ends o... Read More »
Source: http://wiki.answers.com/Q/What_is_TA_cloning_vector
What is a cloning vector?
a carrier used to clone a gene and transfer it from organism to another. Read More »
Source: http://wiki.answers.com/Q/What_is_a_cloning_vector
Answers to Other Common Questions
cloning vectors are the carriers of a particular characteristic. it can be inserted in the non coding region of the DNA and then replicated to yield a modified population. for example; production of insulin from e.coli bacteria. in its non ... Read More »
Source: http://wiki.answers.com/Q/What_are_the_roles_of_cloning_vectors
there are many different vectores as: 1-plasmid system 2-bacteria phage lamda 3-cosmids 4-bacterio artificial system 5-puc system Read More »
Source: http://wiki.answers.com/Q/What_are_the_2_types_of_vectors
( ′plaz·mid ′klōn·iŋ ′vek·tər ) (genetics) A plasmid that accepts foreign deoxyribonucleic acid (DNA) and is therefore used in recombinant DNA experiments. Read More »
Source: http://www.answers.com/topic/plasmid-cloning-vector
The good cloning vector should have small size, high copy number, own origin of replication, restriction sites for many restriction enzymes and selectable markers. Read More »
Source: http://wiki.answers.com/Q/What_a_good_cloning_vector_should_have
In P1 artificial chromosomes (PAC) vectors you can add about 100-300 kb of foreign DNA. Read More »
Source: http://wiki.answers.com/Q/What_is_the_insert_capacity_of_PACs_vec...
The insert capacity of a cosmid vector is about 35-45 kb. Read More »
Source: http://wiki.answers.com/Q/What_is_the_insert_capacity_of_a_cosmid...
Cloning vectors r used to increase the no. of copies of cloned gene or to amplify a foreign gene while expression vectors r used to increase the expression of the foreign gene product. Read More »
Source: http://wiki.answers.com/Q/What_is_the_difference_between_cloning_...
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