Topic: What Is the Bradford Colorimetric Assay
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What are the advantages and disadvantages of Bradford protein ass...
Advantages: Compatible with most buffers, reagents and preparations. One step procedure that is sensitive and accurate. Disadvantages: Non linear correlation over large range of protein concentrations. The dye binds to surface of cuvettes s... Read More »
Source: http://wiki.answers.com/Q/Advantages_and_disadvantages_Bicinchoni...
How do you do a colorimetric MTT assay?
usually in a 96-well plate, using a plate reader. We do the more stable WST-8. Try looking here: http://www.protocol-online.org/ Their fora are like Y!A for professionals. Read More »
Source: http://answers.yahoo.com/question/index?qid=20070809115303AAL99AI
How do I make a colorimetric iron assay?
you go to northwestern? haha Read More »
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What Is the Bradford Colorimetric Assay
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you need to make sure that your assay is linear under the conditions you use (you get a linear response on your spec. and you don't saturate the detector) make sure that other products don't interfere with the colorimetric assay.
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No idea but this might help. http://www.google.co.in/url?sa=t&rct=j&q…
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I think coomassie blue
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the detergents interupt the cross-link hygarious bonds betwwen the molecule chains making up pitahgriotic(-8,-9) polymers
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Source: http://uk.answers.yahoo.com/question/index?qid=20081005171119AABu...
Bradford protein assay is based on the interaction of Coomassie blue with basic and aromatic amino acids - a complex is made resulting in the shift of the absorbance spectrum of Coomassie blue. Detergents (like SDS) will alter the equilibri...
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Source: http://answers.yahoo.com/question/index?qid=20070907081411AAFM1ee
Beer's Law breaks down at high concentrations. Dilute samples give a reaction on the linear portion of the curve. One of the disadvantages of the assay is that it has only a fifty- to one-hundred-fold working range.
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