Topic: You Used Acid alcohol as the Decolorizing Agent
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Answers to Common Questions
Why is alcohol used as a decolorizing agent instead of ethyl alco...
Alcohol is a term used for any O-H group that is attached to a carbon. The general alcohol (the one in liquor) is typically ethanol which is also the same thing as ethyl alcohol. So either these are the same, or you need to be more specific... Read More »
Source: http://wiki.answers.com/Q/Why+acid+alcohol+rather+used+than+ethyl...
What is the decolorizing agent in acid fast stain?
the decolorising agent in acid fast stain is 20%sulphuric acid. Read More »
Source: http://wiki.answers.com/Q/What_is_the_decolorizing_agent_in_acid_...
Why cannot we used ethyl alcohol instead of acid alcohol as decol...
acid alcohol is used for decolorizing is actually for the non-acid fasgt so that they will not retain the carbolfuschin. if you use ethyl alcohol, even the non-acidfast will look acid fast becuase it is inadequate to remove the carbolfuschi... Read More »
Source: http://answers.yahoo.com/question/index?qid=20070629191729AAW3H68
More Common Questions
Answers to Other Common Questions
Answer Some decolorizing of the sample would take place but not as much as using the acid alcohol. Acid alcohol is stronger because it contains hydrochloric acid.
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Source: http://wiki.answers.com/Q/What_is_laparoscopy_Why_it_is_done_Proc...
Nothing. 95% alcohol does essentially the same thing as acid alcohol for this type of staining technique.
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Source: http://www.answerbag.com/q_view/484648
Citric acid is found in citrus fruits. It is a weak organic acid that can be used to flavor or preserve foods. Scientists have used citric acid as a buffering agent in solutions to adjust the pH of the solution and fix that pH so that it ca...
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Source: http://www.ehow.com/how_7376050_use-citric-acid-buffering-agent.h...
alcohol will remove the lipid layer of the G- cells and the outer membrane.
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Source: http://wiki.answers.com/Q/What_would_happen_if_you_decolorized_a_...
Carbolfuchsin will stain the endospore and the vegetative cell pink, the acid alcohol will wash the stain out of the cell but not the spore, then the nigrosin counterstain will stain the background dark as in a negative stain. So, you will ...
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Source: http://www.answerbag.com/q_view/1842617
For the gram stain it is alcohol (ethanol or methanol) or acetone. "When a decolorizer such as alcohol or acetone is added, it interacts with the lipids of the cell membrane. A Gram-negative cell will lose its outer membrane and the peptido...
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Source: http://answers.yahoo.com/question/index?qid=20080305100915AArM5qo
Acid alcohol destains non-acid fast bacteria but not Mycobacteria, which are resistant to the procedure due to the presence of mycolic acid. In the Ziehl Neelsen procedure, Mycobacteria remain red from the carbolfuchsin primary stain after ...
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Source: http://wiki.answers.com/Q/Why_acid_alcohol_is_used_instead_of_eth...
