Chromatography is used in many different disciplines of science to separate mixtures by flowing it over or through a material referred to as a stationary phase. The mixture that is to be separated has to be dissolved in a mobile phase liquid before it can be passed through the other material.Know More
The mixture’s various components move at different speeds through the stationary phase. This is due to every element and molecule having a different weight. A good example of a stationary phase is a piece of paper soaked with the mobile phase, or the liquid. When the paper dries or develops, you can see the various parts of the mixture as spots. The stationary phase in chromatography can be paper, glass coated in an absorbent, or even gas. The devices used to hold the various phases come in several shapes, but are not random and each have a process tied to it. An example of one of these devices is a column chromatography bed, meaning that the stationary bed is in a tube.
In some situations, chromatography is used as a method of purification. This is referred to as preparative chromatography. Another use of chromatography is analytical chromatography. This method of separation occurs with smaller amounts of material and is used to measure analytes in a mixture. The amounts that are measured are only relative proportions, but are accurate enough for scientific testing.Learn more about Chem Lab
Gas liquid chromatography involves the vaporization and injection of a sample onto the top of the chromatographer's column. After that, the sample goes through the column as a result of the flow of mobile gases, while the column itself has a stationary liquid phase that adsorbs onto an inert solid surface. At this point, several different detectors can be used, yielding different sorts of selectivity.Full Answer >
Several different methods can be used to separate mixtures, and the method chosen depends on the nature of the mixture to be separated. To separate a mixture of an insoluble substance from a liquid, you need a container with a hole in the bottom, filtration material such as charcoal or gravel, and a larger container to capture the liquid.Full Answer >
A micropipette is used to transfer small volumes of liquids in chemical, biological and medical laboratories. Pressing on a plunger button at the top of the micropipette will pull the liquid in, and a second press will dispense it. An adjustable micropipette uses a circular volume adjustment knob at the top of the plunger button to determine the amount of liquid that will be drawn in.Full Answer >
A deflagration spoon is used in chemistry experiments when the burning of phosphorus, sulfur or another substance is required. It is capable of withstanding high temperatures and is equipped with features to facilitate working in this environment, such as a long handle angled to keep heat away from the hand.Full Answer >