Chromatography is used in many different disciplines of science to separate mixtures by flowing it over or through a material referred to as a stationary phase. The mixture that is to be separated has to be dissolved in a mobile phase liquid before it can be passed through the other material.Know More
The mixture’s various components move at different speeds through the stationary phase. This is due to every element and molecule having a different weight. A good example of a stationary phase is a piece of paper soaked with the mobile phase, or the liquid. When the paper dries or develops, you can see the various parts of the mixture as spots. The stationary phase in chromatography can be paper, glass coated in an absorbent, or even gas. The devices used to hold the various phases come in several shapes, but are not random and each have a process tied to it. An example of one of these devices is a column chromatography bed, meaning that the stationary bed is in a tube.
In some situations, chromatography is used as a method of purification. This is referred to as preparative chromatography. Another use of chromatography is analytical chromatography. This method of separation occurs with smaller amounts of material and is used to measure analytes in a mixture. The amounts that are measured are only relative proportions, but are accurate enough for scientific testing.Learn more about Chem Lab
Types of chromatography are different methods of separating mixtures under laboratory conditions. There are about a dozen types of chromatography, but five are used most frequently: adsorption chromatography, partition chromatography, ion exchange chromatography, molecular exclusion chromatography and affinity chromatography.Full Answer >
Spatulas are used for measuring solids, moving objects and scraping material out of beakers. Spatulas can be used for more purposes as well, including slipcovers and weights.Full Answer >
Chromatography separates the components of a mixture based on their affinity for a stationary or mobile phase. Each compound leaves the system at a different rate. The quicker a compound is flushed through, the lower its affinity for the stationary phase.Full Answer >
Several different methods can be used to separate mixtures, and the method chosen depends on the nature of the mixture to be separated. To separate a mixture of an insoluble substance from a liquid, you need a container with a hole in the bottom, filtration material such as charcoal or gravel, and a larger container to capture the liquid.Full Answer >