An electron microscope has a higher resolution than a light microscope, which results in much finer detail of the specimen being viewed. Although an electron microscope can magnify a specimen up to 2 million times, a light microscope is advantageous when viewing live cells.Know More
Electron microscopy uses an electron beam rather than light to achieve magnification. To do this, electrons pass through a thin piece of the specimen. The wavelengths of an electron beam are much smaller than light, allowing for better resolution. Because of its ability to reach such acute levels of resolution, it is possible to see the shape of a specimen. Although the finite detail is beneficial in many circumstances, the specimen viewed under an electron microscope must be fixed during preparation so that the structure is not disturbed, which means the specimen will not be alive and can only observe its visual characteristics.
Light microscopy functions on the basis of diffraction. To achieve magnification, light either passes through or reflects off of a specimen before it is transmitted through the optical lenses. In certain conditions, live cells and their biological functions, such as food intake and cell division, can be viewed. Viewers can also track proteins and observe cell movement.Learn more about Biology
A diaphragm on a microscope is the piece that enables the user to adjust the amount of light that is focused under the specimen being observed. A diaphragm is typically found on higher-power microscopes versus less expensive or toy models.Full Answer >
In microscopy, the depth of field refers to the range of distance that runs parallel to the optical axis where the specimen can move and still be viewed without negatively affecting the clarity of the object under observation. The depth of field determines the vertical extent of the plane of focus, which is typically measured in microns.Full Answer >
To use a microscope, one moves the revolving turret so that it is at its lowest magnification setting and puts the sample into the stage clips. The focus knobs and the revolving turret are used to focus and magnify the image farther.Full Answer >
Dissecting microscopes are low-magnification microscopes that can focus on a three-dimensional object that is too thick to be viewed in a regular compound microscope. Dissecting microscopes are also called stereo microscopes as they are designed to allow three-dimensional objects to be seen in three dimensions.Full Answer >