Q:

Why is the field of view brighter under low power?

A:

In light microscopy, lower magnification objective lenses are further from the specimen and survey a larger area, meaning more light enters the microscope, explains How Stuff Works. More magnification means the lens is closer to the stage, which also means less light enters the microscope, making the field of view dimmer.

A light microscope works by shining a light up through objects, such as cells, on a slide. The light passes through the objects up into a small, spherical objective lens, up through the body of the microscope, through a second lens and finally into the eye of the microscope user. By this time, the image on the slide has been magnified many times.

Most light microscopes have a variety of lenses that allow a user to see objects under different magnifications. Each lens is a different magnification and a different distance from the stage. Lower magnification lenses are further from the stage, meaning they observe a larger area and allow in more light. Higher magnification lenses are much closer to the stage. In fact, at the highest resolutions, the lens is separated from the specimen by just a thin layer of oil. Since the lens is so close, the user sees a much smaller area, and less light enters the microscope and the eye. The differing amounts of light reaching the eye make the field of view brighter or dimmer.

Learn More

Related Questions

  • Q:

    What is the depth of field in microscopy?

    A:

    In microscopy, the depth of field refers to the range of distance that runs parallel to the optical axis where the specimen can move and still be viewed without negatively affecting the clarity of the object under observation. The depth of field determines the vertical extent of the plane of focus, which is typically measured in microns.

    Full Answer >
    Filed Under:
  • Q:

    What is the total magnification formula?

    A:

    To determine the total magnification of an image viewed through a microscope, multiply the power of the eyepiece or ocular lens by the power of the objective lens. If the magnification power of the ocular lens is 10x and that of the objective lens is 4x, total magnification is 40x.

    Full Answer >
    Filed Under:
  • Q:

    What does a microscope do?

    A:

    A microscope is an instrument that magnifies objects, enabling visibility of 1,000 times or more than what can be seen by the naked human eye. Microscopes of various types assist researchers with medical diagnostics, and they have aided scientists in attaining knowledge of biology and material science.

    Full Answer >
    Filed Under:
  • Q:

    How do you field dress a deer?

    A:

    Field dress a deer by cutting out the innards in order to keep the meat untainted. This takes a minimum of 10 minutes. You need a knife.

    Full Answer >
    Filed Under:

Explore