Gas liquid chromatography involves the vaporization and injection of a sample onto the top of the chromatographer's column. After that, the sample goes through the column as a result of the flow of mobile gases, while the column itself has a stationary liquid phase that adsorbs onto an inert solid surface. At this point, several different detectors can be used, yielding different sorts of selectivity.Know More
It is important for the carrier gas to be inert chemically, and gases that are frequently used include helium, nitrogen, carbon dioxide and argon; the specific choice generally depends on the type of detector in use.
The sample should enter the column as a vapor "plug," as a slower injection of larger samples weakens resolution and broadens the band. Most commonly, a microsyringe injects sample into a vaporizer port through a rubber septum at the column head.
To create precise outcomes, it is important to control temperatures within the column within tenths of a degree, but the optimal temperature varies with the sample's boiling point. A temperature just above the average excess of the sample yields elution time ranging between two or three minutes to half an hour. Samples that have a wide boiling range tend to respond well to temperature programming.Learn more about Chem Lab
Chromatography is used in many different disciplines of science to separate mixtures by flowing it over or through a material referred to as a stationary phase. The mixture that is to be separated has to be dissolved in a mobile phase liquid before it can be passed through the other material.Full Answer >
A Vigreux column is a piece of scientific equipment that is used for condensing hot vapors. It consists of a straight glass column with walls that are studded with special indentations. This piece of equipment is normally used in chemistry experiments involving fractional distillations or for separating two parts of a mixture based on their boiling points.Full Answer >
Magnetic stirrers use a motor to rotate an internal magnet very quickly, which attracts and pulls a metal stir stick into a circular motion to stir a liquid. Some stirrers use stationary electromagnets instead of a magnet on a motor.Full Answer >
The word decant means to pour or otherwise extract only liquid from a container that holds both liquid and solid particles. In chemistry, this term is often used in experiment instructions.Full Answer >