Q:

Why is it necessary to heat fix bacterial smears prior to staining?

A:

Heat fixing bacterial smears kills the bacterial cells so that they are fixed in place and ready for staining. Heat fixing also adheres the bacterial cells to the slide and allows the sample to take up the stain more easily.

Fixation is a critical step in preparing slides and samples in microbiology, pathology and histology. Fixing bacteria or another type of sample to a slide ensures that the sample will not decay over time and also terminates any type of chemical or biological reactions that could occur on the slide. It preserves cells and tissue samples and allows them to be studied through staining methods at a later date.

Learn More

Related Questions

  • Q:

    Why does nigrosin not stain bacterial cells?

    A:

    The cells of most bacterial cells carry a negative charge that repels nigrosin. This microbiological stain is acidic and readily gives up hydrogen ions to become negatively charged. Nigrosin colors the neutral glass of the microscope slides but does not stain the cells, causing them to show up as a clear spot against a dark background, according to Microbugs.

    Full Answer >
    Filed Under:
  • Q:

    What is the bacterial cell arrangement of E. coli?

    A:

    E. coli bacteria have a single cell arrangement, according to Schenectady County Community College. E. coli is a gram-negative bacillus bacteria that can live with or without oxygen. Escherichia coli is normally found amongst the gut flora of the human intestinal tract and is non-pathogenic.

    Full Answer >
    Filed Under:
  • Q:

    How Is Breathing Related to Cellular Respiration?

    A:

    Cellular respiration is directly related to breathing, as breathing provides the necessary oxygen molecules for the process of cellular respiration to take place; cellular respiration is a process by which cells acquire energy. The oxygen provided by breathing is used as a final hydrogen acceptor for the process, explains Antranik.org.

    Full Answer >
    Filed Under:
  • Q:

    What is the primary function of a mesophyll cell in a plant leaf?

    A:

    The mesophyll cells in a plant leaf play a vital role in photosynthesis by enabling the gas exchange portion of the process, and through the actions of their specialized chlorophyll-containing organelles, called chloroplasts, in which the primary photosynthetic reactions take place. Because they are surrounded by intercellular air pockets, the mesophyll cells are able to perform a gas exchange when the stomatal pores in the leaf's epidermis open. The Greek term "mesophyll" means "middle leaf," and it reflects the mesophyll cells' location between the leaf's thinner and much stiffer upper and lower epidermal layers.

    Full Answer >
    Filed Under:

Explore