The principle of electrophoresis states that in the presence of an electric field, a charged particle moves toward the region of an opposite charge. When the particle has unequal charge distribution in its chemical bonds, it aligns on the electric potential.Know More
An electrode is any conducting material that creates an electrical field to allow current to pass through. The positive end of an electrode is called an anode while the negative end is called a cathode. When a negatively charged particle travels along an electric field, it tends to migrate toward the anode and move against frictional force. The bigger the particle, the slower it moves.
In the fields of molecular biology and biochemistry, electrophoresis is a useful analytical technique where macromolecules of varying sizes and densities are separated. Complex proteins and nucleic acids that undergo electrophoresis move through a gel matrix that is primarily composed of polymerized agarose or polyacrylamide. Agarose is a polysaccharide that forms a gelatin-like substance when dissolved in boiling water. Polyacrylamide is a type of solid gel created by polymerization of acrylamide solutions through the addition of ammonium persulfate coupled with tetramethylenediamine.
Agarose gel is mainly used for separating complex protein molecules and DNA or RNA fragments. Larger molecules get trapped against the porous material while smaller particles pass through easily. Modern researchers prefer the use of polyacrylamide gel. Other forms of gel electrophoresis include isoelectric focusing, 2D electrophoresis, capillary electrophoresis and western blotting.Learn more about Chemistry
Colored ions indicate the presence of a transition metal. Transition metals display colors because of their incompletely filled d orbitals. As the transition metal absorbs a particular wavelength of light to raise the electron orbital, it causes the light that passes through the solution to emit a color.Full Answer >
Rust is the product of a chemical reaction in which iron oxidizes in the presence of an electrolyte. In order to form iron oxide, exposed iron must bond with oxygen, which usually happens when the iron surface gets wet.Full Answer >
Arsenic occurs naturally in the environment and is often found within the Earth's crust, extending its presence into soil, water and rock. Arsenic is also forced into the air during certain chemical processes, causing environmental contamination. Arsenic is a poisonous substance, classified as a metalloid.Full Answer >
Tertiary alcohols do not undergo oxidation in the presence of an oxidizing agent. This is because the carbon atom that contains the alcohol functional group is devoid of any hydrogen atoms, which are necessary in the oxidation mechanism of alcohols.Full Answer >