DNA ligase is a specific type of enzyme, a ligase, (EC 188.8.131.52) that facilitates the
joining of DNA strands together by catalyzing the formation of a phosphodiester ...
This is accomplished by covalently connecting the sugar backbone of the two
DNA fragments. This reaction, called ligation, is performed by the T4 DNA ligase
Ligation of DNA is a central step in many modern molecular biology workflows.
DNA ligases catalyze the formation of a phosphodiester bond between the 3' ...
Ligation Protocol with T4 DNA Ligase (M0202). Protocols.io also provides an
interactive version of this protocol where you can discover and share
Oct 20, 1999 ... Joining linear DNA fragments together with covalent bonds is called ligation.
More specifically, DNA ligation involves creating a phosphodiester ...
Dec 5, 2014 ... Understanding the basic (and simple!) chemistry behind DNA ligation will help
you get better DNA ligation results. Learn all about it here.
The smallest known ATP-dependent DNA ligase is the one from the
bacteriophage T7 (at 41KdA). Eukaryotic DNA ligases may be much larger (
human DNA ...
Jan 27, 2014 ... Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in
three steps. Learn more about DNA ligation at ...
Restriction digestion. Sticky ends and blunt ends. Ligation reactions.
Nov 20, 2007 ... Cells naturally carry out ligation during DNA replication, when the Okazaki
fragments are joined together. Cells also use ligation to repair DNA ...