In molecular biology, DNA ligase is a specific type of enzyme, a ligase, (EC 6.5.
1.1) that facilitates the joining of DNA strands together by catalyzing the formation
This is accomplished by covalently connecting the sugar backbone of the two
DNA fragments. This reaction, called ligation, is performed by the T4 DNA ligase
Ligation of DNA is a central step in many modern molecular biology workflows.
DNA ligases catalyze the formation of a phosphodiester bond between the 3' ...
Oct 20, 1999 ... Joining linear DNA fragments together with covalent bonds is called ligation.
More specifically, DNA ligation involves creating a phosphodiester ...
Dec 5, 2014 ... DNA ligase (EC 188.8.131.52) covalently joins the phosphate backbone of DNA with
blunt or compatible cohesive ends (see Figure 1) and it's natural ...
The smallest known ATP-dependent DNA ligase is the one from the
bacteriophage T7 (at 41KdA). Eukaryotic DNA ligases may be much larger (
human DNA ...
Nov 20, 2007 ... Cells naturally carry out ligation during DNA replication, when the Okazaki
fragments are joined together. Cells also use ligation to repair DNA ...
In molecular biology, ligation refers to the joining of two DNA fragments through
the formation of a phosphodiester bond. An enzyme known as a ligase...
Jul 1, 2012 ... DNA ligation is the process of joining together two DNA molecule ends (either
from the same or different molecules). Specifically, it involves ...
Jan 27, 2014 ... Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in
three steps. Learn more about ligation with our quick animation.