In molecular biology, DNA ligase is a specific type of enzyme, a ligase, (EC 6.5.
1.1) that facilitates the joining of DNA strands together by catalyzing the formation
This is accomplished by covalently connecting the sugar backbone of the two
DNA fragments. This reaction, called ligation, is performed by the T4 DNA ligase
The smallest known ATP-dependent DNA ligase is the one from the
bacteriophage T7 (at 41KdA). Eukaryotic DNA ligases may be much larger (
human DNA ...
Dec 5, 2014 ... DNA ligase (EC 188.8.131.52) covalently joins the phosphate backbone of DNA with
blunt or compatible cohesive ends (see Figure 1) and it's natural ...
Jan 27, 2014 ... Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in
three steps. Learn more about ligation with our quick animation.
In molecular biology, ligation refers to the joining of two DNA fragments through
the formation of a phosphodiester bond. An enzyme known as a ligase...
Cloning by restriction enzyme digestion and ligation is a simple and easy way of
moving a fragment of double-stranded DNA from one plasmid to another.
Guide to DNA ligation and all of the steps that precede it which can affect its
Jul 1, 2012 ... DNA ligation is the process of joining together two DNA molecule ends (either
from the same or different molecules). Specifically, it involves ...
DNA ligase reconnects broken DNA strands, and is used to engineer
recombinant DNA. DNA ligase from bacteriophage T6 (top) and bacteria (bottom)