In molecular biology, DNA ligase is a specific type of enzyme, a ligase, (EC 6.5.
1.1) that facilitates the joining of DNA strands together by catalyzing the formation
This is accomplished by covalently connecting the sugar backbone of the two
DNA fragments. This reaction, called ligation, is performed by the T4 DNA ligase
The smallest known ATP-dependent DNA ligase is the one from the
bacteriophage T7 (at 41KdA). Eukaryotic DNA ligases may be much larger (
human DNA ...
Dec 5, 2014 ... Understanding the basic (and simple!) chemistry behind DNA ligation will help
you get better DNA ligation results. Learn all about it here.
Jan 27, 2014 ... Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in
three steps. Learn more about ligation with our quick animation.
Cloning by restriction enzyme digestion and ligation is a simple and easy way of
moving a fragment of double-stranded DNA from one plasmid to another.
In molecular biology, ligation refers to the joining of two DNA fragments through
the formation of a phosphodiester bond. An enzyme known as a ligase...
Sep 17, 2012 ... This video channel is developed by Amrita University's CREATE http://www.
amrita.edu/create ▷ Subscribe ...
The Fast-Link™ DNA Ligation Kits provide reagents optimized for rapid and ...
Storage Buffer: Fast-Link DNA Ligase is supplied in a 50% glycerol solution ...
The LigaFast™ Rapid DNA Ligation System is designed for the efficient ligation
of sticky-ended DNA inserts into plasmid vectors in just 5 minutes (blunt-ended ...