In molecular biology, DNA ligase is a specific type of enzyme, a ligase, (EC 6.5.
1.1) that facilitates the joining of DNA strands together by catalyzing the formation
This is accomplished by covalently connecting the sugar backbone of the two
DNA fragments. This reaction, called ligation, is performed by the T4 DNA ligase
Ligation of DNA is a central step in many modern molecular biology workflows.
DNA ligases catalyze the formation of a phosphodiester bond between the 3' ...
DNA ligation is commonly used in molecular cloning projects to physically join a
DNA vector to a gene of interest. The ends of the DNA fragments can be blunt or
Nov 20, 2007 ... Cells naturally carry out ligation during DNA replication, when the Okazaki
fragments are joined together. Cells also use ligation to repair DNA ...
The smallest known ATP-dependent DNA ligase is the one from the
bacteriophage T7 (at 41KdA). Eukaryotic DNA ligases may be much larger (
human DNA ...
Dec 5, 2014 ... Understanding the basic (and simple!) chemistry behind DNA ligation will help
you get better DNA ligation results. Learn all about it here.
Jan 27, 2014 ... Ligation, the process of joining DNA fragments with a DNA ligase, proceeds in
three steps. Learn more about DNA ligation at ...
Sep 17, 2012 ... This video channel is developed by Amrita University's CREATE http://www.
amrita.edu/create ▷ Subscribe ...
In molecular biology, ligation refers to the joining of two DNA fragments through
the formation of a phosphodiester bond. An enzyme known as a ligase...