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Is there any easy method for the estimation of hydroxyl radical ...


Aug 18, 2014 ... ... FeCl3 (1:1 v/v), 100µl 1.0mM H2O2 and 100 µl 1.0mm ascorbic acid ,was ... Stock solutions of EDTA (1 mM), FeCl3 (10 mM), Ascorbic Acid (1 ... Hydroxyl radical scavenging assay can be performed by Klein et al and Halliwell methods. ... was measured according to the modified method of Halliwell et al.

Genesis and development of DPPH method of antioxidant assay


Feb 25, 2011 ... On mixing DPPH solution with a substance that can donate a hydrogen .... The modified method introduced of Brand-Williams et al. ... vs. amount of reductant added, for the constant-volume colorimetric titration of DPPH with cysteine hydrochloride<sup>(5)</sup> ..... Halliwell B. Oxidative stress, nutrition and health.

Antioxidant, DNA protective efficacy and HPLC analysis of Annona ...


Feb 25, 2014 ... 2007; Gunjan et al. ... (1/10 dilution) and 2 ml of 7.5 % Na2CO3 (w/v) solution were added and incubated ..... In: Aruoma OI, Halliwell B, editors.

antioxidant activity..

www.doc-developpement-durable.org/file/Arbres-Fruitiers/FICHES_ARBRES/Morinda citrifolia-noni/antioxidant activity of ethanolic root extract of Morinda Citrifolia.pdf

Ranju Pal et al. Annals of ... of sodium carbonate solution (20%) were added sequentially to the test tube. ... 90, 100 in 99.9% ethanol (v/v)]. ... Halliwell et al.

Antioxidant Activities and Polyphenolic Contents of ... - MDPI.com


Feb 10, 2011 ... published. Öztürk et al. tested various extracts of M. juliana for their antioxidant activity by different assays. ... (50:30:10:10, v/v/v/v) as mobile phase; NST/PEG spray reagent; UV light at 365 nm. Tracks: Mc - .... In this assay, the yellow colour of the test solution changes to ..... as described by Halliwell et a...

Radical scavenging and antioxidant activity of tannic acid


Then, the solution which contains 15 μg/mL concentration of tannic acid .... <sup>+</sup> radical scavenging activity was determined according to method of Re et al ..... Fe <sup>2+</sup> ion is the most powerful pro-oxidant among the various species of metal ions ( Halliwell and Gutteridge, 1984). ..... Gülçin, V. Mshvildadze, A. Gepdiremen, R. Elias.

Scavenging of hydroxyl radicals but not of peroxynitrite by inhibitors ...


Feb 10, 2009 ... However, a recent study published in this journal (Dikshit et al., 1996) raised ... of ascorbate, Fe<sup>3+</sup>-EDTA and H2O2 essentially as described by Halliwell et al. ... ( 1987); 1 ml each of 1% (w/v) thiobarbituric acid in 50 mM NaOH and of ... Then 100 μl of the tyrosine solution together with 100 μl of compound to .....

Polyphenolic extract of Termitomyces heimii: antioxidant activity and ...


Aug 1, 2015 ... We used the method described by Halliwell et al. ... of ThePre (0.3–1.0 mg/ml) were added to 2 ml of 0.004 % methanol solution of DPPH (w/v).

Standardized extract of Dicksonia sellowiana Presl. Hook ...


The methanolic solution of DPPH (100 μM, 1 mL) was prepared and added ... was assessed as previously described (Nishikimi et al., 1972), with minor modifications. ... In the end of the incubation TBARS assay was performed ( Halliwell, 2007). .... in 200 μL of pyridine–acetic anhydride (1:1, v/v) and heated at 100 °C for 1 h.

Using microelectrodes - The University of Texas at Dallas


Glass microelectrodes are usually filled with a salt solution. ... for a complete treatment of the subject (Rall & Shepherd, 1968; Hubbard et al. 1969;.

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(Alam et al., 2008a, Alam et al., 2008b, Saha et al., 2008.), in this ... solution ( 0.004% w/v) was prepared in 95% methanol. .... Aruoma OI and Halliwell B (1987 ).

In Vitro Antioxidant Activities of the Fractions of Coccinia Grandis L ...


Oct 27, 2007 ... About 0.3 mM solution of DPPH in 100% ethanol was prepared and 1 ml of this ... system (Fenton reaction) (Halliwell et al., 1987; Ilavarasan et al., 2005). ... (50– 800 µg/ml) in buffer, 200 µl of 1.04 mM EDTA and 200 µM FeCl3 (1:1v/v), .... All the determinations were carried out in triplicate (Ng et al., 2003).

In vitro antioxidant and free radical scavenging activity of different ...


Oct 30, 2015 ... The extraction was performed according to Alam et al. ... The assay is based on the reduction of Mo(VI) to Mo(V) by samples and ... A solution of 0.1 mM DPPH in methanol was prepared, and 2.4 mL of ... Hydroxyl radical scavenging activity of the extractives was determined by the method of Halliwell et al.