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en.wikipedia.org/wiki/Pulsed-field_gel_electrophoresis

Pulsed field gel electrophoresis is a technique used for the separation of large ... and the fact that the DNA does not move in a straight line through the gel.

www.cdc.gov/pulsenet/pathogens/pfge.html

Feb 16, 2016 ... Does PulseNet use other fingerprinting methods? ... How does PFGE work? ... If so, these database managers will work with the public health ...

www.bio-rad.com/en-us/applications-technologies/pulsed-field-gel-electrophoresis

PFGE stemmed from the observation that DNA molecules elongate upon application of an electric field and return to an ...

www.ncbi.nlm.nih.gov/pubmed/17406630

This protocol describes pulsed-field gel electrophoresis (PFGE), a method developed for separation of large DNA molecules. Whereas standard DNA gel ...

www.nature.com/protocolexchange/protocols/1198

Mar 29, 2007 ... This protocol describes pulsed-field gel electrophoresis (PFGE), a method ... using intact chromosomal DNA molecules or working with very large .... and forming chops does not break DNA, and this procedure is included.

bitesizebio.com/29971/pulsed-field-gel-electrophoresis

How PFGE Works. Similar to a standard electrophoresis procedure, DNA is pulled through a PFGE gel due to electric charge. In principle, the apparatus itself is ...

www.sciencelearn.org.nz/videos/825-pulsed-field-gel-electrophoresis

Jun 18, 2008 ... ESR Scientist Dr Brent Gilpin explains how pulsed field gel electrophoresis is used to separate very large fragments of DNA. DNA from an organism is digested with an enzyme and run on a gel, ... Work out the relative ages of some rock . .... And what this does… from an organism which may have 5 million ...

foodsafety.foodscience.cornell.edu/laboratory-molecular-typing-lmt/pulsed-field-gel-electrophoresis-pfge-typing

Note: Standard protocols for Salmonella and E. coli require 1 enzyme, while Listeria requires two enzymes; hence the cost for PFGE typing Salmonella or E. coli ...

www.applied-maths.com/applications/pulsed-field-gel-electrophoresis-pfge-typing

Pulsed-field gel electrophoresis (PFGE) is a highly discriminative molecular typing technique that is used in epidemiological studies worldwide. PFGE is based ...