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Pulsed field gel electrophoresis is a technique used for the separation of large ... and the fact that the DNA does not move in a straight line through the gel.


Feb 16, 2016 ... Pulsed-field gel electrophoresis (PFGE) is a laboratory technique used by scientists to produce a DNA fingerprint for ... How does PFGE work?


PFGE stemmed from the observation that DNA molecules elongate upon application of an electric field and return to an ...


How PFGE Works. Similar to a standard electrophoresis procedure, DNA is pulled through a PFGE gel due to electric charge. In principle, the apparatus itself is ...


This protocol describes pulsed-field gel electrophoresis (PFGE), a method developed for separation of large DNA molecules. Whereas standard DNA gel ...


Mar 29, 2007 ... This protocol describes pulsed-field gel electrophoresis (PFGE), a method ... using intact chromosomal DNA molecules or working with very large .... and forming chops does not break DNA, and this procedure is included.


Jun 18, 2008 ... So pulse field gel electrophoresis involves extracting the DNA from your ... And what this does… from an organism which may have 5 million ...


May 13, 2015 ... How does PulseNet work? ... Detect foodborne disease case clusters by PFGE, thereby facilitating early identification of common source ...


Pulsed-field gel electrophoresis (PFGE) is a highly discriminative molecular typing technique that is used in epidemiological studies worldwide. PFGE is based ...