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1 : 16000 in phosphate buffered saline plus tween 0.05% v/v. ... Plus Western Blotting Detection Reagent; GE Healthcare,. Uppsala, Sweden). Brunetto et al.

The Shc Family Protein Adaptor, Rai, Negatively Regulates T Cell ...


Dec 29, 2011 ... Sakai R, Henderson JT, O'Bryan JP, Elia AJ, Saxton TM, et al. The mammalian ... Pelicci G, Troglio F, Bodini A, Melillo RM, Pettirossi V, et al.

Post-transcriptional regulation of Rbm5 expression in ... - NCBI


Dec 10, 2015 ... ... and (c) RBM10 is important to spermatid differentiation (O'Bryan et al.2013). .... secondary antibody (1:20,000, sc-2005, Santa Cruz Biotechnologies Inc.) and a ... ECL Western Blotting Detection Reagents (GE Healthcare, Mississauga, ..... Bacon ML, Tessier JJ, Rintala-Maki ND, Tang V, Sutherland LC.

The poly(A)-binding protein partner Paip2a controls translation ...


Aug 25, 2010 ... RNA samples were separated on 4.5% acrylamide–50% (w/v) urea gel using ... RNAs were electroblotted onto an Amersham Hybond-N (GE Healthcare, RPN303B). ... with RNase H (New England BioLabs Inc.) in the presence or absence of .... Mali P, et al. .... Khaleghpour K, et al. .... O'Bryan MK, et al.

The Ubiquitin-interacting Motif Protein, S5a, Is Ubiquitinated by All ...


Free iodine was removed with a PD10 desalting column (GE Healthcare). ... A. Ubiquitination of S5a by APC was assayed as described by Kirkpatrick et al. ... pH 7.0, 125 mm potassium acetate, 5 mm EGTA, 0.5% (v/v) Triton X-100, and 1 m m ..... Timsit, Y. E., Miller, S. L., Mohney, R. P., and O'Bryan, J. P. (2005) Biochem.

Antimicrobial Effect and Mode of Action of Terpeneless Cold ... - NCBI


Mar 20, 2012 ... 2011) and Campylobacter (Nannapaneni et al. ... vancomycin intermediate- resistant strains 13136 p<sup>−</sup>m<sup>+</sup> V20 (Pfeltz et al. ... (O'Bryan et al. ... on sterile TSA ( Difco Laboratories, Inc.) by dipping a sterile cotton swab into the culture. ... Cy3 or Cy5 mono-functional NHS ester cyanogen dyes (GE Healthcare, ...

Telomere length dynamics in human memory T cells specific for ...


Aug 26, 2013 ... O'Bryan et al.; licensee BioMed Central Ltd. 2013 ..... PBMC were separated using Ficoll-PaquePlus (GE Healthcare) or Histopaque-1077 (Sigma). ..... Sharma S, Liu J, Bostik V: Long-lived poxvirus immunity, robust CD4 help, ...

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Multistep Phosphorylation by Oncogenic Kinases Enhances the ...


GST fusion proteins were detected with GST Ab (GE Healthcare, Uppsala, ... Roche Ltd, Basel, Switzerland) and incubated for 48 hours before analysis. ..... Curto M, Yaktine A, Pruitt WM, Saotome I, O'Bryan JP, Gupta V, Ratner N, Der CJ, et al.