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en.wikipedia.org/wiki/CDNA_library

A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into ... Similarly, tissue-specific cDNA libraries can be produced. ... Prokaryotes do not have introns in their DNA and therefore do not possess any enzymes that ... Following "sticky end" ligation of the insert into the vector the resulting ...

en.wikipedia.org/wiki/EcoRI

EcoRI (pronounced "eco R one") is a restriction endonuclease enzyme isolated from species E. ... Other restriction enzymes, depending on their cut sites, can also leave 3' ... Restriction enzymes, like EcoRI, that generate sticky ends of DNA are often used to cut .... Not logged in; Talk · Contributions · Create account · Log in ...

www.khanacademy.org/science/biology/biotech-dna-technology/dna-cloning-tutorial/a/restriction-enzymes-dna-ligase

Sticky ends and blunt ends. ... If two pieces of DNA have matching ends, ligase can link them to form a single, ... Many restriction enzymes make staggered cuts at or near their recognition sites, ... Not all restriction enzymes produce sticky ends.

www.ncbi.nlm.nih.gov/books/NBK21881

Importantly, restriction enzymes do not cut randomly; rather, they cut at specific DNA ... enzymes make staggered cuts that create single-stranded sticky ends ...

www.ncbi.nlm.nih.gov/books/NBK21498

Cleavage of human DNA with restriction enzymes that produce about one cut for every ... DNA (e.g., plasmids) that does not integrate into the host-cell chromosome. .... Restriction Fragments with Complementary “Sticky Ends” Are Ligated Easily ... Many other restriction enzymes make similar cuts, generating fragments that ...

www.tusculum.edu/faculty/home/ivanlare/html/genetics/cloning.doc

500 copies ... (a) This will create sticky ends and separate the left and right arms from the middle DNA ... enzyme as that used to cut the lambda genome, so the sticky ends will be complimentary ... (a) The A3 gene is not compatible with Col1b plasmid ... b) The host cell will make many copies of the DNA molecules including ...

www.mrothery.co.uk/genetech/genetechnotes.htm

These sticky ends will stick (or anneal) to another piece of DNA by ... Restriction enzymes are produced naturally by bacteria as a defence against viruses ... Vectors are large molecules which do not readily cross cell membranes, .... This mRNA can be isolated from these cells and used to make cDNA of the insulin gene.

web.mit.edu/7.01x/7.012/documents/7012pset4ansposted.pdf

Oct 27, 2006 ... NdeI and XbaI are the only two enzymes that can excise the gene from the .... plasmid and a digested yeast gene with complementary sticky ends, you don't have ... primers will not be replicated in this reaction; instead, the DNA farther to the left ... make sure to label the 5' and 3' ends of that one strand.

www.bio.utexas.edu/faculty/sjasper/bio212/biotech.html

Under suitable conditions, the bacterial clone will make the protein encoded by the foreign gene. Uses of ... Restriction enzymes leave sticky ends. These will ...