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Confocal microscopy - Wikipedia


Confocal microscopy, most frequently confocal laser scanning microscopy ( CLSM), is an optical ..... The objective was a simple lens with a focal length of 8.5 mm. As opposed to all earlier and most ...

How does a confocal microscope work? - Physics


This web page explains how a confocal microscope works; I've tried to make this explanation not too technical, although for certain parts I've included some ...

A practical guide for fluorescent confocal microscopy | The Marder Lab


A practical guide for fluorescent confocal microscopy by Dirk Bucher This page is not intended to give a complete ... Image resolution and dimensions, part 1.

Working Distance and Parfocal Length | MicroscopyU


The parfocal length is the distance between the specimen plane and the shoulder of ... Microscope objectives are generally designed with a short free working ...

Chapter 2 Confocal Microscopy


Confocal Microscopy. This Chapter offers a brief introduction to confocal microscopy and to other ... Figure 2.2: Scheme of infinite tube-length microscope optics.

Confocal scanning microscopes - Zeiss


A confocal microscope scans a sample sequentially point by point and line by line to ... and gain sensitivity and 1.7× higher resolution in all three dimensions.

Laser Systems for Confocal Microscopy - Olympus Microscopy ...


The lasers commonly employed in laser scanning confocal microscopy are ... If the path length or coherence state of the laser beam fluctuates at a faster interval  ...



Sep 19, 2005 ... The size and shape of this point in 3 dimensions determines the ... The optics of a confocal microscope are arranged such that the fluorescent ...

Confocal microscopy using variable-focal-length microlenses and ...


Appl Opt. 2005 Oct 1;44(28):5928-36. Confocal microscopy using variable-focal- length microlenses and an optical fiber bundle. Yang L(1), Mac Raighne A, ...

The Intermediate Optical System of Laser-Scanning Confocal ...


The basic optical layout of a confocal fluorescence microscope is found in Figure .... of a lens. fi are the focal lengths of the lenses (see Table 9.1). Any conjugate ...

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Confocal optical microscopy - University of California, Berkeley


Confocal optical microscopy is a technique for increasing the contrast of microscope ... It has been typical to make microscope tube lengths 160 mm from the.

Confocal Microscope Objectives - Olympus Microscopy Resource ...


It is possible that specific requirements in confocal microscopy will lead to the .... operates by translating an internal lens group to alter the objective focal length.

Aberrations in Confocal Microscopy | MicroscopyU


Unfortunately, the exacting optical requirements of confocal microscopy are often .... Since the correction for spherical aberration depends on the length of the ...