a microscope that utilizes the phase differences of light rays transmitted by different portions of an object to create an image in which the details of the object are distinct despite their near-uniformity of refractive index.
Phase-contrast microscopy is an optical-microscopy technique that converts
phase shifts in light passing through a transparent specimen to brightness
Phase contrast microscopy, first described in 1934 by Dutch physicist Frits
Zernike, is a contrast-enhancing optical technique that can be utilized to produce
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In a light microscope in bright field mode, light from highly refractive structures
bends farther ... Phase contrast is preferable to bright field microscopy when high
Phase contrast microscopy imparts contrast to unstained biological material by
transforming phase differences of light caused by differences in refractive index ...
Jun 9, 2011 ... Phase contrast is an optical contrast technique for making unstained phase
objects (e.g. flat cells) visible under the optical microscope.
Jun 1, 2012 ... The examination of unstained biological specimens with light microscopy
methods often suffers from poor contrast and, hence, poor visibility of ...
Zernike succeeded in devising a method--now known as Phase Contrast
microscopy--for making unstained, phase objects yield contrast images as if they