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en.wikipedia.org/wiki/Pulsed-field_gel_electrophoresis

Pulsed field gel electrophoresis is a technique used for the separation of large deoxyribonucleic acid (DNA) molecules by applying to a gel matrix an electric field that periodically changes direction. Contents. [hide]. 1 Historical background ; 2 Procedure; 3 Theory; 4 Applications; 5 References; 6 External links. Historical  ...

www.cdc.gov/pulsenet/pathogens/pfge.html

Oct 24, 2017 ... How does PFGE work? The scientist takes bacterial cells from an agar plate. The scientist mixes bacterial cells with melted agarose and pours into a plug mold. The bacterial cells are broken open with biochemicals, or lysed, so that the DNA is free in the agarose plugs. The scientist loads the DNA gelatin ...

www.bio-rad.com/en-us/applications-technologies/pulsed-field-gel-electrophoresis

? PFGE stemmed from the observation that DNA molecules elongate upon application of an electric field and return to an unelongated state upon removal of the electric field; this relaxation rate is dependent on the size of the DNA. When the orientation of the electric field is changed ...

www.ncbi.nlm.nih.gov/pubmed/17406630

Nat Protoc. 2007;2(3):677-84. Pulsed-field gel electrophoresis. Herschleb J(1), Ananiev G, Schwartz DC. Author information: (1)Department of Chemistry, UW Biotechnology Center, University of Wisconsin-Madison, 425 Henry Mall, Madison, Wisconsin 53706, USA. This protocol describes pulsed-field gel electrophoresis ...

www.ncbi.nlm.nih.gov/pmc/articles/PMC4740595

Jan 1, 2016 ... Pulsed-Homogeneous Orthogonal Field Gel Electrophoresis (PHOGE): The difference among this method and other methods of PFGE with homogeneous fields is the orientation angle of 90 degrees in field. DNA molecules are moved four times per cycle instead of twice. DNA lines do not follow a straight ...

bitesizebio.com/29971/pulsed-field-gel-electrophoresis

How PFGE Works. Similar to a standard electrophoresis procedure, DNA is pulled through a PFGE gel due to electric charge. In principle, the apparatus itself is essentially the same as a standard electrophoresis unit. Similar to standard electrophoresis, there are electrodes that allow the electric charge to pass through the ...

www.ask.com/youtube?q=How+Does+Pulsed+Field+Gel+Electrophoresis+Work&v=rc0BfOAM5j4
Jun 21, 2015 ... This lecture explains about the pulsed field gel electrophoresis process also known as the PFGE that helps in the separation of large DNA fragments with bett. .. ... 1 question : why do we need to use a gel (plug) to digest DNA using restriction enzyme? Why dont we just put a DNA suspension + restriction ...

www.sciencelearn.org.nz/videos/825-pulsed-field-gel-electrophoresis

Jun 18, 2008 ... So pulse field gel electrophoresis involves extracting the DNA from your pathogenic organism and digesting it, or cutting it up into fragments with a rare- cutting restriction enzyme. And what this does… from an organism which may have 5 million base pairs, you get 15 to 20 fragments, which if you separate ...

www.applied-maths.com/applications/pulsed-field-gel-electrophoresis-pfge-typing

Pulsed-field gel electrophoresis (PFGE) is a highly discriminative molecular typing technique that is used in epidemiological studies worldwide. PFGE is based upon the variable migration of large DNA restriction fragments in an electrical field of alternating polarity. By comparing the fingerprints of any two isolates, one can ...