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en.wikipedia.org/wiki/Ampicillin_resistance

Ampicillin resistance is bacterial resistance to the antibiotic ampicillin. Many strains of bacteria are resistant to a variety of antibiotics, however the resistance that is most commonly used in research and industry is ampicillin resistance. It is used as a selectable marker in bacterial transformation. ... All the cells that lack the amp resistance gene will be killed off, leaving only ...

www.easynotecards.com/notecard_set/72750

1) The following are steps used to make DNA fingerprints. ... 9) If you have inserted a gene in the Ti plasmid, the next step in genetic engineering is .... 36) The use of an antibiotic-resistance gene on a plasmid used in genetic engineering ...

www.addgene.org/mol-bio-reference/plasmid-background

Plasmids are fragments of double-stranded DNA that can be used to study and ... have been optimized for their use of studying and manipulating genes. ... which includes a DNA replication origin, an antibiotic-resistance gene, and a region in ... Self-replicating - Once you have constructed a plasmid, you can easily make an ...

www.ncbi.nlm.nih.gov/books/NBK21881

The recombinant DNA mixture is then used to transform bacterial cells, and it is common ... With the use of such methods, the bulk of DNA extracted from the donor will be ... Plasmids such as those carrying genes for resistance to the antibiotic ... Recognition, Cleavage, and Modification Sites of Various Restriction Enzymes ...

www.khanacademy.org/science/biology/biotech-dna-technology/dna-cloning-tutorial/a/bacterial-transformation-selection

Science & engineering ... Colonies with the right plasmid can be grown to make large cultures of ... Then, we can use antibiotic selection and DNA analysis methods to identify ... Plasmids used in cloning contain an antibiotic resistance gene. .... in a recent gene therapy clinical trial for patients with the genetic disorder cystic ...

www.ncbi.nlm.nih.gov/books/NBK21498

Two types of vectors are most commonly used: E. coli plasmid vectors and ... Many naturally occurring plasmids contain genes that provide some benefit to the host ... evolved, making their host cells resistant to a variety of different antibiotics ... for their use in DNA cloning: a replication origin, a drug-resistance gene, and a  ...

www.khanacademy.org/science/biology/biotech-dna-technology/dna-cloning-tutorial/a/overview-dna-cloning

Bacteria with the correct plasmid are used to make more plasmid DNA or, in some .... A plasmid typically contains an antibiotic resistance gene, which allows bacteria to .... often use DNA cloning to build artificial, recombinant versions of genes that help ..... Subjects; Math by subject · Math by grade · Science & engineering ...

www.sciencelearn.org.nz/resources/2032-bacterial-transformation

Nov 16, 2007 ... Bacteria are commonly used as host cells for making copies of DNA in ... For example, bacteria can acquire DNA that makes them resistant to antibiotics. ... DNA, called plasmids, which have a much smaller number of genes.

mrsmillersblog.files.wordpress.com/2010/09/a2-5-2-3-genetic-engineering.pptx

Recombinant DNA = A section of DNA, often in the form of a plasmid, which is ... mRNA from transcription can be used as a template to make a copy of the gene; Gene ... gene into cattle; Insert insulin gene into bacteria for diabetic humans to use ... Original plasmids have antibiotic resistance gene (used as a genetic marker) ...